|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Mouse WFDC2 ORF mammalian expression plasmid, C-GFPSpark tag||MG50715-ACG|
|Mouse WFDC2 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50715-ACR|
|Mouse WFDC2 ORF mammalian expression plasmid, C-Flag tag||MG50715-CF|
|Mouse WFDC2 ORF mammalian expression plasmid, C-His tag||MG50715-CH|
|Mouse WFDC2 ORF mammalian expression plasmid, C-Myc tag||MG50715-CM|
|Mouse WFDC2 ORF mammalian expression plasmid, C-HA tag||MG50715-CY|
|Mouse WFDC2 Gene cDNA clone plasmid||MG50715-G|
|Mouse WFDC2 ORF mammalian expression plasmid, N-Flag tag||MG50715-NF|
|Mouse WFDC2 ORF mammalian expression plasmid, N-His tag||MG50715-NH|
|Mouse WFDC2 ORF mammalian expression plasmid, N-Myc tag||MG50715-NM|
|Mouse WFDC2 ORF mammalian expression plasmid, N-HA tag||MG50715-NY|
|Mouse WFDC2 natural ORF mammalian expression plasmid||MG50715-UT|
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WAP four-disulfide core domain protein 2, also known as Epididymal secretory protein E4, Major epididymis-specific protein E4, Putative protease inhibitor WAP5, WFDC2 and HE4, is a secreted protein which contains two WAP domains. WFDC2 / HE4 is a member of a family of stable 4-disulfide core proteins that are secreted at high levels. It is expressed in a number of normal tissues, including male reproductive system, regions of the respiratory tract and nasopharynx. It is highly expressed in a number of tumors cells lines, such ovarian, colon, breast, lung and renal cells lines. Initially described as being exclusively transcribed in the epididymis. WFDC2 may be a component of the innate immune defences of the lung, nasal and oral cavities and suggest that WFDC2 functions in concert with related WAP domain containing proteins in epithelial host defence. WFDC2 re-expression in lung carcinomas may prove to be associated with tumour type and should be studied in further detail. Mammary gland expression of tammar WFDC2 during the course of lactation showed WFDC2 was elevated during pregnancy, reduced in early lactation and absent in mid-late lactation. WFDC2 / HE4 can undergo a complex series of alternative splicing events that can potentially yield five distinct WAP domain containing protein isoforms.