|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Mouse IFNG ORF mammalian expression plasmid, C-GFPSpark tag||MG50709-ACG|
|Mouse IFNG ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50709-ACR|
|Mouse IFNG ORF mammalian expression plasmid, C-Flag tag||MG50709-CF|
|Mouse IFNG ORF mammalian expression plasmid, C-His tag||MG50709-CH|
|Mouse IFNG ORF mammalian expression plasmid, C-Myc tag||MG50709-CM|
|Mouse IFNG ORF mammalian expression plasmid, C-HA tag||MG50709-CY|
|Mouse IFNG Gene cDNA clone plasmid||MG50709-M|
|Mouse IFNG ORF mammalian expression plasmid, N-Flag tag||MG50709-NF|
|Mouse IFNG ORF mammalian expression plasmid, N-His tag||MG50709-NH|
|Mouse IFNG ORF mammalian expression plasmid, N-Myc tag||MG50709-NM|
|Mouse IFNG ORF mammalian expression plasmid, N-HA tag||MG50709-NY|
|Mouse IFNG natural ORF mammalian expression plasmid||MG50709-UT|
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IFN gamma, also known as IFNG, is a secreted protein which belongs to the type I I interferon family. IFN gamma is produced predominantly by natural killer and natural killer T cells as part of the innate immune response, and by CD4 and CD8 cytotoxic T lymphocyte effector T cells once antigen-specific immunity develops. IFN gamma has antiviral, immunoregulatory, and anti-tumor properties. IFNG, in addition to having antiviral activity, has important immunoregulatory functions, it is a potent activator of macrophages, and has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons. The IFNG monomer consists of a core of six α-helices and an extended unfolded sequence in the C-terminal region. IFN gamma is critical for innate and adaptive immunity against viral and intracellular bacterial infections and for tumor control. Aberrant IFN gamma expression is associated with a number of autoinflammatory and autoimmune diseases. The importance of IFN gamma in the immune system stems in part from its ability to inhibit viral replication directly, and most importantly from its immunostimulatory and immunomodulatory effects. IFNG also promotes NK cell activity.