|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein.
Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems.
|Human NGFR ORF mammalian expression plasmid, C-GFPSpark tag||HG13184-ACG|
|Human NGFR ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG13184-ACR|
|Human NGFR ORF mammalian expression plasmid, C-Flag tag||HG13184-CF|
|Human NGFR ORF mammalian expression plasmid, C-His tag||HG13184-CH|
|Human NGFR ORF mammalian expression plasmid, C-Myc tag||HG13184-CM|
|Human NGFR ORF mammalian expression plasmid, C-HA tag||HG13184-CY|
|Human NGFR Gene cDNA clone plasmid||HG13184-G|
|Human NGFR ORF mammalian expression plasmid, N-Flag tag||HG13184-NF|
|Human NGFR ORF mammalian expression plasmid, N-His tag||HG13184-NH|
|Human NGFR ORF mammalian expression plasmid, N-Myc tag||HG13184-NM|
|Human NGFR ORF mammalian expression plasmid, N-HA tag||HG13184-NY|
|Human NGFR natural ORF mammalian expression plasmid||HG13184-UT|
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Nerve growth factor receptors (NGFRs) belong to a large growth factor receptor family. NGFR includes two types of receptors: high-affinity nerve growth factor receptor and low-affinity nerve growth factor receptor. High-affinity nerve growth factor receptor is also referred as Trk familywhose members are bound by some neurotrophins with high affinity. Nerve growth factor binds with TrkA after being released from target cells, the NGF / TrkA complex is subsequently trafficked back to the cell body. The Low-affinity nerve growth factor receptor also named p75 which binds with all kinds of neurotrophins with low affinity. All the four kinds of neurotrophins, including Nerve growth factor, Brain derived neurotrophic factor, Neurotrophin-3, and Neurotrophin-4 bind to the p75. Studies have proved that NGFR acts as a molecular signal swith that determines cell death or survival by three steps. First, pro-nerve growth factor (prNGF) triggers cell apoptosis by its high affinity binding to p75NTR, while NGF induces neuronal survival with low-affinity binding. Second, p75NTR mediates cell death by combining with co-receptor sortilin, whereas it promotes neuronal survival through combination with proNGF. Third, release of the intracellular domain chopper or cleavage short p75 NTR can independently initiate neuronal apoptosis.