|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.
|Human CTNNB1 ORF mammalian expression plasmid, C-GFPSpark tag||HG11279-ACG|
|Human CTNNB1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG11279-ACR|
|Human CTNNB1 ORF mammalian expression plasmid, N-GFPSpark tag||HG11279-ANG|
|Human CTNNB1 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||HG11279-ANR|
|Human CTNNB1 ORF mammalian expression plasmid, C-Flag tag||HG11279-CF|
|Human CTNNB1 ORF mammalian expression plasmid, C-His tag||HG11279-CH|
|Human CTNNB1 ORF mammalian expression plasmid, C-Myc tag||HG11279-CM|
|Human CTNNB1 ORF mammalian expression plasmid, C-HA tag||HG11279-CY|
|Human CTNNB1 Gene cDNA clone plasmid||HG11279-M|
|Human CTNNB1 natural ORF mammalian expression plasmid, HA tag||HG11279-M-Y|
|Human CTNNB1 ORF mammalian expression plasmid, N-Flag tag||HG11279-NF|
|Human CTNNB1 ORF mammalian expression plasmid, N-His tag||HG11279-NH|
|Human CTNNB1 ORF mammalian expression plasmid, N-Myc tag||HG11279-NM|
|Human CTNNB1 ORF mammalian expression plasmid, N-HA tag||HG11279-NY|
|Human CTNNB1 natural ORF mammalian expression plasmid||HG11279-UT|
|Learn more about expression Vectors|
beta-Catenin, also known as CTNNB1, is a member of the armadillo family of proteins. These proteins have multiple copies of the so-called armadillo repeat domain, which is specialized for protein-protein binding. It is part of a complex of proteins that constitute adherens junctions (AJs). AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. CTNNB1 also anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally, beta-Catenin binds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. Defects in beta-Catenin can cause colorectal cancer, pilomatrixoma (PTR), medulloblastoma, and ovarian cancer. CTNNB1 is a key dowstream component of the canonical Wnt signaling pathway. In the absence of Wnt, it forms a complex with AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome. In the presence of Wnt ligand, beta-Catenin is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, leading to activate Wnt responsive genes. CTNNB1 is involved in the regulation of cell adhesion. The majority of beta-catenin is localized to the cell membrane and is part of E-cadherin/catenin adhesion complexes which are proposed to couple cadherins to the actin cytoskeleton.