|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Mouse EREG ORF mammalian expression plasmid, C-GFPSpark tag||MG50599-ACG|
|Mouse EREG ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50599-ACR|
|Mouse EREG ORF mammalian expression plasmid, C-Flag tag||MG50599-CF|
|Mouse EREG ORF mammalian expression plasmid, C-His tag||MG50599-CH|
|Mouse EREG ORF mammalian expression plasmid, C-Myc tag||MG50599-CM|
|Mouse EREG ORF mammalian expression plasmid, C-HA tag||MG50599-CY|
|Mouse EREG Gene cDNA clone plasmid||MG50599-M|
|Mouse EREG ORF mammalian expression plasmid, N-Flag tag||MG50599-NF|
|Mouse EREG ORF mammalian expression plasmid, N-His tag||MG50599-NH|
|Mouse EREG ORF mammalian expression plasmid, N-Myc tag||MG50599-NM|
|Mouse EREG ORF mammalian expression plasmid, N-HA tag||MG50599-NY|
|Mouse EREG natural ORF mammalian expression plasmid||MG50599-UT|
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Epiregulin (EREG) is a member of the epidermal growth factor family. Epiregulin (EREG) can function as a ligand of EGFR (epidermal growth factor receptor), as well as a ligand of most members of the ERBB (v-erb-b2 oncogene homolog) family of tyrosine-kinase receptors. Epiregulin (EREG) exhibit bifunctional regulatory properties: it inhibit the growth of several epithelial tumor cells and stimulated the growth of fibroblasts and various other types of cells. Epiregulin (EREG) bound to the EGF receptors of epidermoid carcinoma A431 cells much more weakly than did EGF, but was nevertheless much more potent than EGF as a mitogen for rat primary hepatocytes and Balb/c 3T3 A31 fibroblasts. These findings suggest that epiregulin (EREG) plays important roles in regulating the growth of epithelial cells and fibroblasts by binding to receptors for EGF-related ligands. Epiregulin (EREG) is the broadest specificity EGF-like ligand so far characterized: not only does it stimulate homodimers of both ErbB-1 and ErbB-4, it also activates all possible heterodimeric ErbB complexes.