|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Mouse HSPD1 ORF mammalian expression plasmid, C-GFPSpark tag||MG50596-ACG|
|Mouse HSPD1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50596-ACR|
|Mouse HSPD1 ORF mammalian expression plasmid, N-GFPSpark tag||MG50596-ANG|
|Mouse HSPD1 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||MG50596-ANR|
|Mouse HSPD1 ORF mammalian expression plasmid, C-Flag tag||MG50596-CF|
|Mouse HSPD1 ORF mammalian expression plasmid, C-His tag||MG50596-CH|
|Mouse HSPD1 ORF mammalian expression plasmid, C-Myc tag||MG50596-CM|
|Mouse HSPD1 ORF mammalian expression plasmid, C-HA tag||MG50596-CY|
|Mouse HSPD1 Gene cDNA clone plasmid||MG50596-M|
|Mouse HSPD1 ORF mammalian expression plasmid, N-Flag tag||MG50596-NF|
|Mouse HSPD1 ORF mammalian expression plasmid, N-His tag||MG50596-NH|
|Mouse HSPD1 ORF mammalian expression plasmid, N-Myc tag||MG50596-NM|
|Mouse HSPD1 ORF mammalian expression plasmid, N-HA tag||MG50596-NY|
|Mouse HSPD1 Gene cDNA clone plasmid||MG50596-U|
|Mouse HSPD1 natural ORF mammalian expression plasmid||MG50596-UT|
|Learn more about expression Vectors|
HSPD1, also known as HSP60, is a member of the chaperonin family. HSPD1 may function as a signaling molecule in the innate immune system. This protein is essential for the folding and assembly of newly imported proteins in the mitochondria. It may also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix. HSPD1 gene is adjacent to a related family member and the region between the 2 genes functions as a bidirectional promoter. Several pseudogenes have been associated with this gene. Mutations associated with this gene cause autosomal recessive spastic paraplegia 13.Defects in HSPD1 are a cause of spastic paraplegia autosomal dominant type 13 (SPG13). Spastic paraplegia is a degenerative spinal cord disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs. Defects in HSPD1 are the cause of leukodystrophy hypomyelinating type 4 (HLD4); also called mitochondrial HSP60 chaperonopathy or MitCHAP-60 disease. HLD4 is a severe autosomal recessive hypomyelinating leukodystrophy. HSPD1 is cinically characterized by infantile-onset rotary nystagmus, progressive spastic paraplegia, neurologic regression, motor impairment, profound mental retardation. Death usually occurrs within the first two decades of life.