|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Mouse EFNB1 ORF mammalian expression plasmid, C-GFPSpark tag||MG50580-ACG|
|Mouse EFNB1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50580-ACR|
|Mouse EFNB1 ORF mammalian expression plasmid, C-Flag tag||MG50580-CF|
|Mouse EFNB1 ORF mammalian expression plasmid, C-His tag||MG50580-CH|
|Mouse EFNB1 ORF mammalian expression plasmid, C-Myc tag||MG50580-CM|
|Mouse EFNB1 ORF mammalian expression plasmid, C-HA tag||MG50580-CY|
|Mouse EFNB1 Gene cDNA clone plasmid||MG50580-M|
|Mouse EFNB1 ORF mammalian expression plasmid, N-Flag tag||MG50580-NF|
|Mouse EFNB1 ORF mammalian expression plasmid, N-His tag||MG50580-NH|
|Mouse EFNB1 ORF mammalian expression plasmid, N-Myc tag||MG50580-NM|
|Mouse EFNB1 ORF mammalian expression plasmid, N-HA tag||MG50580-NY|
|Mouse EFNB1 natural ORF mammalian expression plasmid||MG50580-UT|
|Learn more about expression Vectors|
Ephrin-B1 also known as EFNB1, is a member of the ephrin family. The transmembrane- associated ephrin ligands and their Eph family of receptor tyrosine kinases are expressed by cells of the SVZ. Eph/ephrin interactions are implicated in axon guidance, neural crest cell migration, establishment of segmental boundaries, and formation of angiogenic capillary plexi. Eph receptors and ephrins are divided into two subclasses, A and B, based on binding specificities. Ephrin subclasses are further distinguished by their mode of attachment to the plasma membrane: ephrin-A ligands bind EphA receptors and are anchored to the plasma membrane via a glycosylphosphatidylinositol (GPI) linkage, whereas ephrin-B ligands bind EphB receptors and are anchored via a transmembrane domain. An exception is the EphA4 receptor, which binds both subclasses of ephrins. EphrinB1 and B class Eph receptors provide positional cues required for the normal morphogenesis of skeletal elements. Another malformation, preaxial polydactyly, was exclusively seen in heterozygous females in which expression of the X-linked ephrinB1 gene was mosaic, so that ectopic EphB-ephrinB1 interactions led to restricted cell movements and the bifurcation of digital rays.