|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human BPHL ORF mammalian expression plasmid, C-GFPSpark tag||HG15763-ACG|
|Human BPHL ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG15763-ACR|
|Human BPHL ORF mammalian expression plasmid, C-Flag tag||HG15763-CF|
|Human BPHL ORF mammalian expression plasmid, C-His tag||HG15763-CH|
|Human BPHL ORF mammalian expression plasmid, C-Myc tag||HG15763-CM|
|Human BPHL ORF mammalian expression plasmid, C-HA tag||HG15763-CY|
|Human BPHL Gene cDNA clone plasmid||HG15763-G|
|Human BPHL ORF mammalian expression plasmid, N-Flag tag||HG15763-NF|
|Human BPHL ORF mammalian expression plasmid, N-His tag||HG15763-NH|
|Human BPHL ORF mammalian expression plasmid, N-Myc tag||HG15763-NM|
|Human BPHL ORF mammalian expression plasmid, N-HA tag||HG15763-NY|
|Human BPHL natural ORF mammalian expression plasmid||HG15763-UT|
|Learn more about expression Vectors|
BPHL is a member of the serine protease family. BPHL is expressed large quantities in liver and kidney and in minor quantities in heart, intestine and skeletal muscle. BPHL is a specific alpha-amino acid ester hydrolase that prefers small, hydrophobic, and aromatic side chains and does not have a stringent requirement for the leaving group other than preferring a primary alcohol. It catalyzes the hydrolytic activation of amino acid ester prodrugs of nucleoside analogs such as valacyclovir and valganciclovir. BPHL also activates valacyclovir to acyclovir. It may play a role in detoxification processes.