|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.
|Ferret VCAM1 ORF mammalian expression plasmid, C-GFPSpark tag||FG60134-ACG|
|Ferret VCAM1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||FG60134-ACR|
|Ferret VCAM1 ORF mammalian expression plasmid, C-Flag tag||FG60134-CF|
|Ferret VCAM1 ORF mammalian expression plasmid, C-His tag||FG60134-CH|
|Ferret VCAM1 ORF mammalian expression plasmid, C-Myc tag||FG60134-CM|
|Ferret VCAM1 ORF mammalian expression plasmid, C-HA tag||FG60134-CY|
|Ferret VCAM1 Gene cDNA clone plasmid||FG60134-G|
|Ferret VCAM1 ORF mammalian expression plasmid, N-Flag tag||FG60134-NF|
|Ferret VCAM1 ORF mammalian expression plasmid, N-His tag||FG60134-NH|
|Ferret VCAM1 ORF mammalian expression plasmid, N-Myc tag||FG60134-NM|
|Ferret VCAM1 ORF mammalian expression plasmid, N-HA tag||FG60134-NY|
|Ferret VCAM1 natural ORF mammalian expression plasmid||FG60134-UT|
|Learn more about expression Vectors|
Vascular cell adhesion molecule 1 (VCAM-1), also known as CD106, is a cell surface sialoglycoprotein belonging to the immunoglobulin superfamily. Two forms of VCAM-1 with either six or seven extracellular Ig-like domains are generated by alternative splicing, with the longer form predominant. VCAM-1 is an endothelial ligand for very late antigen-4 (VLA-4) and α4ß7 integrin expressed on leukocytes, and thus mediates leukocyte-endothelial cell adhesion and signal transduction. VCAM-1 expression is induced on endothelial cells during inflammatory bowel disease, atherosclerosis, allograft rejection, infection, and asthmatic responses. During these responses, VCAM-1 forms a scaffold for leukocyte migration. VCAM-1 also activates signals within endothelial cells resulting in the opening of an "endothelial cell gate" through which leukocytes migrate. VCAM-1 has been identified as a potential anti-inflammatory therapeutic target, the hypothesis being that reduced expression of VCAM-1 will slow the development of atherosclerosis. In addition, VCAM-1-activated signals in endothelial cells are regulated by cytokines indicating that it is important to consider both endothelial cell adhesion molecule expression and function during inflammatory processes.