|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Mouse CADM1 ORF mammalian expression plasmid, C-GFPSpark tag||MG50538-ACG|
|Mouse CADM1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50538-ACR|
|Mouse CADM1 ORF mammalian expression plasmid, C-Flag tag||MG50538-CF|
|Mouse CADM1 ORF mammalian expression plasmid, C-His tag||MG50538-CH|
|Mouse CADM1 ORF mammalian expression plasmid, C-Myc tag||MG50538-CM|
|Mouse CADM1 ORF mammalian expression plasmid, C-HA tag||MG50538-CY|
|Mouse CADM1 Gene cDNA clone plasmid||MG50538-G|
|Mouse CADM1 ORF mammalian expression plasmid, N-Flag tag||MG50538-NF|
|Mouse CADM1 ORF mammalian expression plasmid, N-His tag||MG50538-NH|
|Mouse CADM1 ORF mammalian expression plasmid, N-Myc tag||MG50538-NM|
|Mouse CADM1 ORF mammalian expression plasmid, N-HA tag||MG50538-NY|
|Mouse CADM1 natural ORF mammalian expression plasmid||MG50538-UT|
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Members of the immunoglobulin superfamily often play key roles in intercellular adhesion. IGSF4 is a novel immunoglobulin (Ig)-like intercellular adhesion molecule. Three Ig-like domains are included in the extracellular domain of IGSF4 and mediate homophilic or heterophilic interactions independently of Ca2+. The cytoplasmic domain of IGSF4 contains the binding motifs that connect to actin fibers. Since IGSF4 has been characterized by several independent research groups, this molecule is called by three names, TSLC1, SgIGSF and SynCAM. IGSF4 was first characterized as a tumor suppressor of non-small cell lung cancer and termed TSLC1. It is a single-pass type I membrane protein which belongs to the nectin family, which may be involved in neuronal migration, axon growth, pathfinding, and fasciculation on the axons of differentiating neurons. In addition, CADM1 may play diverse roles in the spermatogenesis including in the adhesion of spermatocytes and spermatids to Sertoli cells and for their normal differentiation into mature spermatozoa. In neuroblastoma, loss of CADM1 expression has recently been found in disseminated tumours with adverse outcome, prompting us to investigate its role in neuroblastoma tumour progression. The downregulation of CADM1 tumour suppressor gene expression is a critical event in neuroblastoma pathogenesis resulting in tumour progression.