|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Mouse CD99 ORF mammalian expression plasmid, C-GFPSpark tag||MG50520-ACG|
|Mouse CD99 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50520-ACR|
|Mouse CD99 ORF mammalian expression plasmid, C-Flag tag||MG50520-CF|
|Mouse CD99 ORF mammalian expression plasmid, C-His tag||MG50520-CH|
|Mouse CD99 ORF mammalian expression plasmid, C-Myc tag||MG50520-CM|
|Mouse CD99 ORF mammalian expression plasmid, C-HA tag||MG50520-CY|
|Mouse CD99 Gene cDNA clone plasmid||MG50520-M|
|Mouse CD99 ORF mammalian expression plasmid, N-Flag tag||MG50520-NF|
|Mouse CD99 ORF mammalian expression plasmid, N-His tag||MG50520-NH|
|Mouse CD99 ORF mammalian expression plasmid, N-Myc tag||MG50520-NM|
|Mouse CD99 ORF mammalian expression plasmid, N-HA tag||MG50520-NY|
|Mouse CD99 natural ORF mammalian expression plasmid||MG50520-UT|
|Learn more about expression Vectors|
The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD99 is a transmembrane protein expressed on most hematopoietic cells, endothelial cells and at the borders between confluent cells. CD99 is also found expressed in the development of normal ovary and testis as well as in 25 sex cord-stromal tumors, 7 epithelial neoplasms, and 6 germ cell tumors. CD99 may be a useful marker for sex cord-stromal tumors and that its degree of reactivity correlates with the degree of differentiation in Sertoli-Leydig cell tumors. Additionally, CD99 might aid in distinguishing granulose cell tumors of the ovary from poorly differentiated carcinomas and it has been reported to be a sensitive and specific marker for Ewing's sarcoma and primitive neuroectodermal tumor.