|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Mouse CD180 ORF mammalian expression plasmid, C-GFPSpark tag||MG50491-ACG|
|Mouse CD180 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50491-ACR|
|Mouse CD180 ORF mammalian expression plasmid, C-Flag tag||MG50491-CF|
|Mouse CD180 ORF mammalian expression plasmid, C-His tag||MG50491-CH|
|Mouse CD180 ORF mammalian expression plasmid, C-Myc tag||MG50491-CM|
|Mouse CD180 ORF mammalian expression plasmid, C-HA tag||MG50491-CY|
|Mouse CD180 Gene cDNA clone plasmid||MG50491-M|
|Mouse CD180 ORF mammalian expression plasmid, N-Flag tag||MG50491-NF|
|Mouse CD180 ORF mammalian expression plasmid, N-His tag||MG50491-NH|
|Mouse CD180 ORF mammalian expression plasmid, N-Myc tag||MG50491-NM|
|Mouse CD180 ORF mammalian expression plasmid, N-HA tag||MG50491-NY|
|Mouse CD180 natural ORF mammalian expression plasmid||MG50491-UT|
|Learn more about expression Vectors|
The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD180, also known as RP105, is a B-cell surface molecule belonging to the family of pathogen receptors, Toll-like receptors (TLR). CD180 has an extracellular leucine-rich repeats and a short cytoplasmic tail. CD180 / RP105 interact with an extracellular molecule named MD1 and then together form the cell surface receptor complex RP105 / MD1 which induces B-cell activation in humans and mice, leading to proliferation and up-regulation of a costimulatory molecule, B7.2 / CD86. CD180 / RP105 also has a role in LPS response because B cells lacking RP105 show hyporesponsiveness to LPS.