|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Mouse EGF ORF mammalian expression plasmid, C-GFPSpark tag||MG50482-ACG|
|Mouse EGF ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50482-ACR|
|Mouse EGF ORF mammalian expression plasmid, C-Flag tag||MG50482-CF|
|Mouse EGF ORF mammalian expression plasmid, C-His tag||MG50482-CH|
|Mouse EGF ORF mammalian expression plasmid, C-Myc tag||MG50482-CM|
|Mouse EGF ORF mammalian expression plasmid, C-HA tag||MG50482-CY|
|Mouse EGF Gene cDNA clone plasmid||MG50482-M|
|Mouse EGF ORF mammalian expression plasmid, N-Flag tag||MG50482-NF|
|Mouse EGF ORF mammalian expression plasmid, N-His tag||MG50482-NH|
|Mouse EGF ORF mammalian expression plasmid, N-Myc tag||MG50482-NM|
|Mouse EGF ORF mammalian expression plasmid, N-HA tag||MG50482-NY|
|Mouse EGF natural ORF mammalian expression plasmid||MG50482-UT|
|Learn more about expression Vectors|
EGF is the founding member of the EGF-family of proteins. Members of this protein family have highly similar structural and functional characteristics. EGF contains 9 EGF-like domains and 9 LDL-receptor class B repeats. Human EGF is a 6045-Da protein with 53 amino acid residues and three intramolecular disulfide bonds. As a low-molecular-weight polypeptide, EGF was first purified from the mouse submandibular gland, but since then it was found in many human tissues including submandibular gland, parotid gland. It can also be found in human platelets, macrophages, urine, saliva, milk, and plasma. EGF is a growth factor that stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. It results in cellular proliferation, differentiation, and survival. Salivary EGF, which seems also regulated by dietary inorganic iodine, also plays an important physiological role in the maintenance of oro-esophageal and gastric tissue integrity. EGF acts by binding with high affinity to epidermal growth factor receptor on the cell surface and stimulating the intrinsic protein-tyrosine kinase activity of the receptor. The tyrosine kinase activity, in turn, initiates a signal transduction cascade that results in a variety of biochemical changes within the cell - a rise in intracellular calcium levels, increased glycolysis and protein synthesis, and increases in the expression of certain genes including the gene for EGFR - that ultimately lead to DNA synthesis and cell proliferation.