|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Mouse PDGF-C ORF mammalian expression plasmid, C-GFPSpark tag||MG50458-ACG|
|Mouse PDGF-C ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50458-ACR|
|Mouse PDGF-C ORF mammalian expression plasmid, C-Flag tag||MG50458-CF|
|Mouse PDGF-C ORF mammalian expression plasmid, C-His tag||MG50458-CH|
|Mouse PDGF-C ORF mammalian expression plasmid, C-Myc tag||MG50458-CM|
|Mouse PDGF-C ORF mammalian expression plasmid, C-HA tag||MG50458-CY|
|Mouse PDGF-C Gene cDNA clone plasmid||MG50458-M|
|Mouse PDGF-C ORF mammalian expression plasmid, N-Flag tag||MG50458-NF|
|Mouse PDGF-C ORF mammalian expression plasmid, N-His tag||MG50458-NH|
|Mouse PDGF-C ORF mammalian expression plasmid, N-Myc tag||MG50458-NM|
|Mouse PDGF-C ORF mammalian expression plasmid, N-HA tag||MG50458-NY|
|Mouse PDGF-C natural ORF mammalian expression plasmid||MG50458-UT|
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PDGF-C is a member of the PDGF/VEGF family of growth factors with a unique domain organization and expression pattern. Platelet-derived growth factor receptors (PDGFRs) are catalytic receptors that have intracellular tyrosine kinase activity. They have roles in the regulation of many biological processes including embryonic development, angiogenesis, cell proliferation and differentiation, and contribute to the pathophysiology of some diseases, including cancer. There are two isoforms of the PDGFR receptor; PDGFRalpha and PDGFRbeta, which can form homo- or heterodimers. The endogenous PDGFR ligands are PDGF-A, -B, -C and -D, which induce receptor dimerization and transphosphorylation at specific tyrosine residues upon binding. This activates the intracellular kinase activity, initiating intracellular signaling through the MAPK, PI 3-K and PKCgamma pathways. PDGF-C acts as a specific ligand for alpha platelet-derived growth factor receptor homodimer, and alpha and beta heterodimer. Binding of this growth factor to its affinity receptor elicits a variety of cellular responses. PDGF-C Appears to be involved in the three stages of wound healing: inflammation, proliferation and remodeling. Involved in fibrotic processes, in which transformation of interstitial fibroblasts into myofibroblasts plus collagen deposition occurs.