|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Mouse S100A5 ORF mammalian expression plasmid, C-GFPSpark tag||MG50427-ACG|
|Mouse S100A5 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50427-ACR|
|Mouse S100A5 ORF mammalian expression plasmid, N-GFPSpark tag||MG50427-ANG|
|Mouse S100A5 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||MG50427-ANR|
|Mouse S100A5 ORF mammalian expression plasmid, C-Flag tag||MG50427-CF|
|Mouse S100A5 ORF mammalian expression plasmid, C-His tag||MG50427-CH|
|Mouse S100A5 ORF mammalian expression plasmid, C-Myc tag||MG50427-CM|
|Mouse S100A5 ORF mammalian expression plasmid, C-HA tag||MG50427-CY|
|Mouse S100A5 Gene cDNA clone plasmid||MG50427-M|
|Mouse S100A5 ORF mammalian expression plasmid, N-Flag tag||MG50427-NF|
|Mouse S100A5 ORF mammalian expression plasmid, N-His tag||MG50427-NH|
|Mouse S100A5 ORF mammalian expression plasmid, N-Myc tag||MG50427-NM|
|Mouse S100A5 ORF mammalian expression plasmid, N-HA tag||MG50427-NY|
|Mouse S100A5 natural ORF mammalian expression plasmid||MG50427-UT|
|Learn more about expression Vectors|
S100 protein?is a family of low molecular weight protein found in vertebrates characterized by two?EF-hand calcium-binding motifs. There are at least 21 different S100 proteins, and the name is derived from the fact that the protein is?100%?soluble in ammonium sulfate?at neutral?pH. Most S100 proteins are disulfide-linked homodimer, and is normally present in cells derived from the?neural crest, chondrocytes, macrophages, dendritic cells, etc. S100 proteins have been implicated in a variety of intracellular and extracellular functions. They are involved in regulation of protein phosphorylation, transcription factors, the dynamics of cytoskeleton constituents, enzyme activities, cell growth and differentiation, and the inflammatory response.?? Protein S100-A5, also known as Protein S-100D, S100 calcium-binding protein A5, S100A5 and S100D, is a member of the S100 family which contains two?EF-hand domains. S100A5 is also a novel member of the EF-hand superfamily of calcium-binding proteins that is poorly characterized at the protein level. It is expressed in very restricted regions of the adult brain. From birth onwards, S100A5 remained a neuronal-specific protein, only located in a subpopulation of neurons in the spiral ganglion.