|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human TNFSF9 ORF mammalian expression plasmid, C-GFPSpark tag||HG15693-ACG|
|Human TNFSF9 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG15693-ACR|
|Human TNFSF9 ORF mammalian expression plasmid, C-Flag tag||HG15693-CF|
|Human TNFSF9 ORF mammalian expression plasmid, C-His tag||HG15693-CH|
|Human TNFSF9 ORF mammalian expression plasmid, C-Myc tag||HG15693-CM|
|Human TNFSF9 ORF mammalian expression plasmid, C-HA tag||HG15693-CY|
|Human TNFSF9 Gene cDNA clone plasmid||HG15693-G|
|Human TNFSF9 ORF mammalian expression plasmid, N-Flag tag||HG15693-NF|
|Human TNFSF9 ORF mammalian expression plasmid, N-His tag||HG15693-NH|
|Human TNFSF9 ORF mammalian expression plasmid, N-Myc tag||HG15693-NM|
|Human TNFSF9 ORF mammalian expression plasmid, N-HA tag||HG15693-NY|
|Human TNFSF9 natural ORF mammalian expression plasmid||HG15693-UT|
|Learn more about expression Vectors|
4-1BBL is the high affinity ligand of 4-1BB, also known as CD137L or TNFSF9. It is shown to be a type II surface glycoprotein belonging to the TNF superfamily. Expression of 4-1BBL is restricted to APCs, such as dendritic cells, macrophages, and activated B cells. Members of the TNF-TNF receptor superfamily have been shown to play critical roles in regulating cellular activation, differentiation and apoptosis. Several studies have reported that 4-1BBL/4-1BB interaction provided a co-stimulatory signal to T cells, and increased T cell proliferation and cytokines production. In additon, 4-1BBL is involved in cancers, infectious diseases and autoimmune diseases.