|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Mouse CPB1 ORF mammalian expression plasmid, C-GFPSpark tag||MG50386-ACG|
|Mouse CPB1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50386-ACR|
|Mouse CPB1 ORF mammalian expression plasmid, C-Flag tag||MG50386-CF|
|Mouse CPB1 ORF mammalian expression plasmid, C-His tag||MG50386-CH|
|Mouse CPB1 ORF mammalian expression plasmid, C-Myc tag||MG50386-CM|
|Mouse CPB1 ORF mammalian expression plasmid, C-HA tag||MG50386-CY|
|Mouse CPB1 Gene cDNA clone plasmid||MG50386-M|
|Mouse CPB1 ORF mammalian expression plasmid, N-Flag tag||MG50386-NF|
|Mouse CPB1 ORF mammalian expression plasmid, N-His tag||MG50386-NH|
|Mouse CPB1 ORF mammalian expression plasmid, N-Myc tag||MG50386-NM|
|Mouse CPB1 ORF mammalian expression plasmid, N-HA tag||MG50386-NY|
|Mouse CPB1 natural ORF mammalian expression plasmid||MG50386-UT|
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Human Carboxypeptidase B1, also well known as pancreatic procarboxypeptidase B (PCPB), is a highly pancreas -specific protein (PASP), and has been identified previously as a serum marker for acute pancreatitis and pancreatic graft rejection. As the prototype for those human exopeptidases that cleave off basic C-terminal residues, CPB1 specifically cleaves the C-terminal Arg and Lys residues with a preference for Arg. The B1 and B2 forms of procarboxypeptidase B differ from each other mainly in isoelectric point.The deduced amino acid sequence of PCPB predicts a 416-amino acid preproenzyme consisting of a 15-aa signal peptide, a 95-aa activation peptide and a 307-aa mature chain. The secreted PCPB zymogen is converted to enzymatically active CPB1 by limited proteolysis by trypsin.