|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Mouse TPO R ORF mammalian expression plasmid, C-GFPSpark tag||MG50373-ACG|
|Mouse TPO R ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50373-ACR|
|Mouse TPO R ORF mammalian expression plasmid, C-Flag tag||MG50373-CF|
|Mouse TPO R ORF mammalian expression plasmid, C-His tag||MG50373-CH|
|Mouse TPO R ORF mammalian expression plasmid, C-Myc tag||MG50373-CM|
|Mouse TPO R ORF mammalian expression plasmid, C-HA tag||MG50373-CY|
|Mouse TPO R Gene cDNA clone plasmid||MG50373-M|
|Mouse TPO R ORF mammalian expression plasmid, N-Flag tag||MG50373-NF|
|Mouse TPO R ORF mammalian expression plasmid, N-His tag||MG50373-NH|
|Mouse TPO R ORF mammalian expression plasmid, N-Myc tag||MG50373-NM|
|Mouse TPO R ORF mammalian expression plasmid, N-HA tag||MG50373-NY|
|Mouse TPO R natural ORF mammalian expression plasmid||MG50373-UT|
|Learn more about expression Vectors|
CD110, also known as c-MPL, is a 635 amino acid transmembrane domain, with two extracellular cytokine receptor domains and two intracellular cytokine receptor box motifs. It is expressed at a low level in a large number of cells of hematopoietic origin. C-MPL is homologous with members of the hematopoietic receptor superfamily. Presence of anti-sense oligodeoxynucleotides of c-mpl inhibited megakaryocyte colony formation. Thrombopoietin is the ligand for c-mpl. It was shown to be the major regulator of megakaryocytopoiesis and platelet formation. Defects in c-MPL are a cause of congenital amegakaryocytic thrombocytopeniawhich is a disease characterized by isolated thrombocytopenia and megakaryocytopenia with no physical anomalies. Defects in c-MPL also cause thrombocythemia type 2 and myelofibrosis with myeloid metaplasia.