|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Mouse CXCL13 ORF mammalian expression plasmid, C-GFPSpark tag||MG50140-ACG|
|Mouse CXCL13 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50140-ACR|
|Mouse CXCL13 ORF mammalian expression plasmid, C-Flag tag||MG50140-CF|
|Mouse CXCL13 ORF mammalian expression plasmid, C-His tag||MG50140-CH|
|Mouse CXCL13 ORF mammalian expression plasmid, C-Myc tag||MG50140-CM|
|Mouse CXCL13 ORF mammalian expression plasmid, C-HA tag||MG50140-CY|
|Mouse CXCL13 Gene cDNA clone plasmid||MG50140-M|
|Mouse CXCL13 ORF mammalian expression plasmid, N-Flag tag||MG50140-NF|
|Mouse CXCL13 ORF mammalian expression plasmid, N-His tag||MG50140-NH|
|Mouse CXCL13 ORF mammalian expression plasmid, N-Myc tag||MG50140-NM|
|Mouse CXCL13 ORF mammalian expression plasmid, N-HA tag||MG50140-NY|
|Mouse CXCL13 natural ORF mammalian expression plasmid||MG50140-UT|
|Learn more about expression Vectors|
The chemokine CXCL13, also known as BCA-1 (B-cell-attracting chemokine-1) or BLC (B-lymphocyte chemoattractant), which belongs to the CXC chemokine family. CXCL13 and its receptor CXCR5 control the organization of B cells within follicles of lymphoid tissues. CXCL13 is known to dictate homing and motility of B cells in lymphoid tissue and has been implicated in the formation of ectopic lymphoid tissue in chronic inflammation. It involves in B-cell compartmental homing within secondary lymphoid organs and recently implicated in the pathogenesis of inflammatory and malignant lymphocyte-mediated diseases. In Primary central nervous system lymphoma (PCNSL), expression of BCA-1 by malignant lymphocytes and vascular endothelium may influence tumor development and localization to central nervous system (CNS). In T-lymphocytes, CXCL13 expression is thought to reflect a germinal center origin of the T-cell. CXCL13 expression may also provide an additional useful tool for the diagnosis of Angioimmunoblastic T-cell lymphoma (AITL).