|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Mouse CTSD ORF mammalian expression plasmid, C-GFPSpark tag||MG50127-ACG|
|Mouse CTSD ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50127-ACR|
|Mouse CTSD ORF mammalian expression plasmid, C-Flag tag||MG50127-CF|
|Mouse CTSD ORF mammalian expression plasmid, C-His tag||MG50127-CH|
|Mouse CTSD ORF mammalian expression plasmid, C-Myc tag||MG50127-CM|
|Mouse CTSD ORF mammalian expression plasmid, C-HA tag||MG50127-CY|
|Mouse CTSD Gene cDNA clone plasmid||MG50127-M|
|Mouse CTSD ORF mammalian expression plasmid, N-Flag tag||MG50127-NF|
|Mouse CTSD ORF mammalian expression plasmid, N-His tag||MG50127-NH|
|Mouse CTSD ORF mammalian expression plasmid, N-Myc tag||MG50127-NM|
|Mouse CTSD ORF mammalian expression plasmid, N-HA tag||MG50127-NY|
|Mouse CTSD natural ORF mammalian expression plasmid||MG50127-UT|
|Learn more about expression Vectors|
Cathepsin D (CTSD), a well known lysosomal aspartyl protease and belongs to the peptidase C1 family, which is a normal and major component of lysosomes, and is found in almost all cells and tissues of mammals. Its mostly described function is intracellular catabolism in lysosomal compartments, other physiological effect include hormone and antigen processing. Cathepsin D has a specificity similar to but narrower than that of pepsin A. Cathepsin D plays an important role in the degradation of proteins, the generation of bioactive proteins, antigen processing, etc. Among different role in cell physiology, a new function of this enzyme is examined. Cathepsin D is an important regulator of apoptotic pathways in cells. It acts at different stage of intrinsic and extrinsic pathway of apoptosis. In addition, CTSD secreted from human prostate carcinoma cells are responsible for the generation of angiostatin, a potent endogenous inhibitor of angiogenesis, suggesting its contribution to the prevention of tumor growth and angiogenesis-dependent growth of metastases.