|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.
|Canine CX3CL1 ORF mammalian expression plasmid, C-GFPSpark tag||DG70071-ACG|
|Canine CX3CL1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||DG70071-ACR|
|Canine CX3CL1 ORF mammalian expression plasmid, C-Flag tag||DG70071-CF|
|Canine CX3CL1 ORF mammalian expression plasmid, C-His tag||DG70071-CH|
|Canine CX3CL1 ORF mammalian expression plasmid, C-Myc tag||DG70071-CM|
|Canine CX3CL1 ORF mammalian expression plasmid, C-HA tag||DG70071-CY|
|Canine CX3CL1 Gene cDNA clone plasmid||DG70071-G|
|Canine CX3CL1 ORF mammalian expression plasmid, N-Flag tag||DG70071-NF|
|Canine CX3CL1 ORF mammalian expression plasmid, N-His tag||DG70071-NH|
|Canine CX3CL1 ORF mammalian expression plasmid, N-Myc tag||DG70071-NM|
|Canine CX3CL1 ORF mammalian expression plasmid, N-HA tag||DG70071-NY|
|Canine CX3CL1 natural ORF mammalian expression plasmid||DG70071-UT|
|Learn more about expression Vectors|
Fractalkine or Chemokine (C-X3-C motif) ligand 1 (CX3CL1) is a member of the CX3C chemokine family. Fractalkine / CX3CL1 is a unique chemokine that functions not only as a chemoattractant but also as an adhesion molecule and is expressed on endothelial cells activated by proinflammatory cytokines, such as interferon-gamma and tumor necrosis factor-alpha. Fractalkine/CX3CL1 is expressed in a membrane-bound form on activated endothelial cells and mediates attachment and firm adhesion of T cells, monocytes and NK cells. Fractalkine / CX3CL1 is associated with dendritic cells (DC) in epidermis and lymphoid organs. The fractalkine receptor, CX3CR1, is expressed on cytotoxic effector lymphocytes, including natural killer (NK) cells and cytotoxic T lymphocytes, which contain high levels of intracellular perforin and granzyme B, and on macrophages. Soluble fractalkine causes migration of NK cells, cytotoxic T lymphocytes, and macrophages, whereas the membrane-bound form captures and enhances the subsequent migration of these cells in response to secondary stimulation with other chemokines.