|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Mouse FetuinA / AHSG ORF mammalian expression plasmid, C-GFPSpark tag||MG50093-ACG|
|Mouse FetuinA / AHSG ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50093-ACR|
|Mouse FetuinA / AHSG ORF mammalian expression plasmid, C-Flag tag||MG50093-CF|
|Mouse FetuinA / AHSG ORF mammalian expression plasmid, C-His tag||MG50093-CH|
|Mouse FetuinA / AHSG ORF mammalian expression plasmid, C-Myc tag||MG50093-CM|
|Mouse FetuinA / AHSG ORF mammalian expression plasmid, C-HA tag||MG50093-CY|
|Mouse FetuinA / AHSG Gene cDNA clone plasmid||MG50093-M|
|Mouse FetuinA / AHSG ORF mammalian expression plasmid, N-Flag tag||MG50093-NF|
|Mouse FetuinA / AHSG ORF mammalian expression plasmid, N-His tag||MG50093-NH|
|Mouse FetuinA / AHSG ORF mammalian expression plasmid, N-Myc tag||MG50093-NM|
|Mouse FetuinA / AHSG ORF mammalian expression plasmid, N-HA tag||MG50093-NY|
|Mouse FetuinA / AHSG natural ORF mammalian expression plasmid||MG50093-UT|
|Learn more about expression Vectors|
Fetuin-A, also known as Alpha-2-HS-Glycoprotein (AHSG), belongs to the Fetuin family, is a plasma binding protein, and is more abundant in fetal than adult blood. It is involved in several functions, such as endocytosis, brain development and the formation of bone tissue. Fetuins are carrier proteins like albumin. Fetuin-A forms soluble complexes with calcium and phosphate and thus is a carrier of otherwise insoluble calcium phosphate. Thus Fetuin-A is a potent inhibitor of pathological calcification. The circulating levels of fetuin-A, a well-described inhibitor of calcification, regulate the cell-dependent process of osteogenesis. The low circulating fetuin-A levels are associated with a greater prevalence and/or severity of Vascular calcification (VC) and increased risk for all-cause and cardiovascular mortality. However, high circulating fetuin-A levels appear to induce insulin resistance and, in non-dialyzed subjects with diabetic nephropathy, are directly related to VC burden. The emerging role of fetuin-A deficiency as a risk factor in dialysis patients was documented in cross-sectional studies demonstrating a significant correlation with all-cause and cardiovascular mortality. Additionally, Human fetuin-A is a negative acute phase protein involved in inflammatory diseases, thus being a potential physiological regulator of meprin activity. Fetuin-A is a broad-range protease inhibitor. Fetuin-A and cystatin C as endogenous proteolytic regulators of meprin activity broadens our understanding of the proteolytic network in plasma.