|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Mouse MBL ORF mammalian expression plasmid, C-GFPSpark tag||MG50063-ACG|
|Mouse MBL ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50063-ACR|
|Mouse MBL ORF mammalian expression plasmid, C-Flag tag||MG50063-CF|
|Mouse MBL ORF mammalian expression plasmid, C-His tag||MG50063-CH|
|Mouse MBL ORF mammalian expression plasmid, C-Myc tag||MG50063-CM|
|Mouse MBL ORF mammalian expression plasmid, C-HA tag||MG50063-CY|
|Mouse MBL Gene cDNA clone plasmid||MG50063-M|
|Mouse MBL ORF mammalian expression plasmid, N-Flag tag||MG50063-NF|
|Mouse MBL ORF mammalian expression plasmid, N-His tag||MG50063-NH|
|Mouse MBL ORF mammalian expression plasmid, N-Myc tag||MG50063-NM|
|Mouse MBL ORF mammalian expression plasmid, N-HA tag||MG50063-NY|
|Mouse MBL natural ORF mammalian expression plasmid||MG50063-UT|
|Learn more about expression Vectors|
MBL (mannose-binding lectin) is primarily a liver-derived collagen-like serum protein, which binds sugar structures on micro-organisms and on dying host cells and is one of the four known mediators that initiate activation of the complement system via the lectin pathway. MBL and the ficolins (Ficolin-1, Ficolin-2 and Ficolin-3) are soluble collagen-like proteins that are involved in innate immune defence. They bind sugar structures or acetylated compounds present on microorganisms and on dying host cells and they initiate activation of the lectin complement pathway in varying degrees. MBL2 encodes the mannose-binding lectin, which is a key player in the innate immune system and has recently been found to play a role in development of type 1 diabetes and gestational diabetes mellitus. Common variant alleles situated both in promoter and structural regions of the MBL2 gene influence the stability and the serum concentration of the protein. Several polymorphisms in the promoter and structural regions of MBL2 adversely affect the plasma concentration and oligomeric state of MBL. The possession of mutant alleles has been linked to disease outcome for a variety of bacterial and viral infections. Mutant MBL2 haplotypes have been linked to disease progression and response to therapy in HCV infection.