|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Mouse SCARB2 ORF mammalian expression plasmid, C-GFPSpark tag||MG50052-ACG|
|Mouse SCARB2 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50052-ACR|
|Mouse SCARB2 ORF mammalian expression plasmid, C-Flag tag||MG50052-CF|
|Mouse SCARB2 ORF mammalian expression plasmid, C-His tag||MG50052-CH|
|Mouse SCARB2 ORF mammalian expression plasmid, C-Myc tag||MG50052-CM|
|Mouse SCARB2 ORF mammalian expression plasmid, C-HA tag||MG50052-CY|
|Mouse SCARB2 Gene cDNA clone plasmid||MG50052-M|
|Mouse SCARB2 ORF mammalian expression plasmid, N-Flag tag||MG50052-NF|
|Mouse SCARB2 ORF mammalian expression plasmid, N-His tag||MG50052-NH|
|Mouse SCARB2 ORF mammalian expression plasmid, N-Myc tag||MG50052-NM|
|Mouse SCARB2 ORF mammalian expression plasmid, N-HA tag||MG50052-NY|
|Mouse SCARB2 natural ORF mammalian expression plasmid||MG50052-UT|
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Lysosomal Integral Membrane Protein II (LIMPII), also known as SCARB2, LPG85, and CD36L2, is a type I II multi-pass membrane glycoprotein that is located primarily in limiting membranes of lysosomes and endosomes on all tissues and cell types so far examined. This protein may participate in membrane transportation and the reorganization of endosomal/lysosomal compartment. LIMPII is identified as a receptor for EV71 (human enterovirus species A, Enterovirus 71) and CVA16 (coxsackievirus A16) which are most frequently associated with hand, foot and mouth disease (HFMD). Expression of human LIMPII enables normally unsusceptible cell lines to support the viruses’ propagation and develop cytopathic effects. In addition, LIMPII also has been shown to bind thrombospondin-1, may contribute to the pro-adhesive changes of activated platelets during coagulation, and inflammation. Deficiency of the protein in mice impairs cell membrane transport processes and causes pelvic junction obstruction, deafness, and peripheral neuropathy.