|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Mouse ADAM9 ORF mammalian expression plasmid, C-GFPSpark tag||MG50044-ACG|
|Mouse ADAM9 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50044-ACR|
|Mouse ADAM9 ORF mammalian expression plasmid, C-Flag tag||MG50044-CF|
|Mouse ADAM9 ORF mammalian expression plasmid, C-His tag||MG50044-CH|
|Mouse ADAM9 ORF mammalian expression plasmid, C-Myc tag||MG50044-CM|
|Mouse ADAM9 ORF mammalian expression plasmid, C-HA tag||MG50044-CY|
|Mouse ADAM9 Gene cDNA clone plasmid||MG50044-M|
|Mouse ADAM9 ORF mammalian expression plasmid, N-Flag tag||MG50044-NF|
|Mouse ADAM9 ORF mammalian expression plasmid, N-His tag||MG50044-NH|
|Mouse ADAM9 ORF mammalian expression plasmid, N-Myc tag||MG50044-NM|
|Mouse ADAM9 ORF mammalian expression plasmid, N-HA tag||MG50044-NY|
|Mouse ADAM9 natural ORF mammalian expression plasmid||MG50044-UT|
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ADAM9 (A disintegrin and metallopeptidase domain 9, MDC9, meltrin gamma), is a type 1 transmembrane protein that has been associated with cancer development and metastases. ADAM9 is consistently overexpressed in various human cancers, and plays a role in tumorigenesis in mouse models. ADAM9 cleaves and releases a number of molecules with important roles in tumorigenesis and angiogenesis, such as EGF, FGFR2iiib, Tie-2, Flk-1, EphB4, CD40, VCAM-1, and VE-cadherin, and could represent a potential therapeutic target in tumors where it is highly expressed. ADAM9 belongs to a family of transmembrane, disintegrin-containing metalloproteinases involved in protein ectodomain shedding and cell-cell and cell-matrix interactions. ADAM-9 adhesive domain plays a role in regulating the motility of cells by interaction with beta1 integrins and modulates MMP synthesis.