|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.
|Canine ANGPT2 ORF mammalian expression plasmid, C-GFPSpark tag||DG70034-ACG|
|Canine ANGPT2 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||DG70034-ACR|
|Canine ANGPT2 ORF mammalian expression plasmid, C-Flag tag||DG70034-CF|
|Canine ANGPT2 ORF mammalian expression plasmid, C-His tag||DG70034-CH|
|Canine ANGPT2 ORF mammalian expression plasmid, C-Myc tag||DG70034-CM|
|Canine ANGPT2 ORF mammalian expression plasmid, C-HA tag||DG70034-CY|
|Canine ANGPT2 Gene cDNA clone plasmid||DG70034-G|
|Canine ANGPT2 ORF mammalian expression plasmid, N-Flag tag||DG70034-NF|
|Canine ANGPT2 ORF mammalian expression plasmid, N-His tag||DG70034-NH|
|Canine ANGPT2 ORF mammalian expression plasmid, N-Myc tag||DG70034-NM|
|Canine ANGPT2 ORF mammalian expression plasmid, N-HA tag||DG70034-NY|
|Canine ANGPT2 natural ORF mammalian expression plasmid||DG70034-UT|
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Angiopoietin-2 (ANG 2, or ANGPT2), is a member of the ANG family, which plays an important role in angiogenesis during the development and growth of human cancers. Both ANGPT-1 and ANGPT-2 appear to bind to the tyrosine kinase receptor, Tie-2, found primarily on the luminal surface of endothelial cells. ANG-2's role in angiogenesis generally is considered as an antagonist for ANG1, inhibiting ANG1-promoted Tie2 signaling, which is critical for blood vessel maturation and stabilization. ANG-2 modulates angiogenesis in a cooperative manner with another important angiogenic factor, vascular endothelial growth factor A. Genetic studies have revealed that ANG-2 also is critical in lymphangiogenesis during development. ANG-2 has multiple physiologic effects that regulate vascular tone, hormone secretion, tissue growth and neural activity. Several reports indicate that ANG-2 can induce neovascularization in experimental systems due to the expression of different growth factors such as angiopoietin 2, vascular endothelial factor, and its receptor, fibroblast growth factor, platelet derived growth factor, transforming growth factor beta and epidermal growth factor. In addition, ANG-2 is strongly expressed in the vasculature of many tumors and it has been suggested that ANG-2 may act synergistically with other cytokines such as vascular endothelial growth factor to promote tumor-associated Angiogenesis and tumor progression.