|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Mouse CD5L ORF mammalian expression plasmid, C-GFPSpark tag||MG50020-ACG|
|Mouse CD5L ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50020-ACR|
|Mouse CD5L ORF mammalian expression plasmid, C-Flag tag||MG50020-CF|
|Mouse CD5L ORF mammalian expression plasmid, C-His tag||MG50020-CH|
|Mouse CD5L ORF mammalian expression plasmid, C-Myc tag||MG50020-CM|
|Mouse CD5L ORF mammalian expression plasmid, C-HA tag||MG50020-CY|
|Mouse CD5L Gene cDNA clone plasmid||MG50020-M|
|Mouse CD5L ORF mammalian expression plasmid, N-Flag tag||MG50020-NF|
|Mouse CD5L ORF mammalian expression plasmid, N-His tag||MG50020-NH|
|Mouse CD5L ORF mammalian expression plasmid, N-Myc tag||MG50020-NM|
|Mouse CD5L ORF mammalian expression plasmid, N-HA tag||MG50020-NY|
|Mouse CD5L natural ORF mammalian expression plasmid||MG50020-UT|
|Learn more about expression Vectors|
CD5L, also known as CD5 antigen-like, is a soluble protein belonging to group B of the scavenger receptor cysteine-rich (SRCR) superfamily and contains three SRCR domains. It is a secreted glycoprotein and expressed by macrophages presentin lymphoid tissues (spleen, lymph node, thymus, and bone marrow). It binds to myelomonocytic and lymphoid cells and may play an important role in the regulation of the innate and adaptive immune systems. CD5L functions as a pattern recognition molecule by binding both lipoteichoic acid (LTA) on Gram positive and lipopolysaccharide (LPS) on Gram negative bacteria. and the SRCR domain 1 of CD5L retains both the LPS and LTA binding activities. In addtion, it is revealed that CD5L seems to play a role as an inhibitor of apoptosis.