|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Mouse ARSA ORF mammalian expression plasmid, C-GFPSpark tag||MG50018-ACG|
|Mouse ARSA ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50018-ACR|
|Mouse ARSA ORF mammalian expression plasmid, C-Flag tag||MG50018-CF|
|Mouse ARSA ORF mammalian expression plasmid, C-His tag||MG50018-CH|
|Mouse ARSA ORF mammalian expression plasmid, C-Myc tag||MG50018-CM|
|Mouse ARSA ORF mammalian expression plasmid, C-HA tag||MG50018-CY|
|Mouse ARSA Gene cDNA clone plasmid||MG50018-M|
|Mouse ARSA ORF mammalian expression plasmid, N-Flag tag||MG50018-NF|
|Mouse ARSA ORF mammalian expression plasmid, N-His tag||MG50018-NH|
|Mouse ARSA ORF mammalian expression plasmid, N-Myc tag||MG50018-NM|
|Mouse ARSA ORF mammalian expression plasmid, N-HA tag||MG50018-NY|
|Mouse ARSA natural ORF mammalian expression plasmid||MG50018-UT|
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Arylsulfatase A (ARSA) is synthesized as a 52KDa lysosomal enzyme. It is a member of the sulfatase family that is required for the lysosomal degradation of cerebroside-3-sulfate, a sphingolipid sulfate ester and a major constituent of the myelin sheet. Arylsulfatase A is activated by a required co- or posttranslational modification with the oxidation of cysteine to formylglycine. Metachromatic leukodystrophy (MLD) is a lysosomal storage disease in the central and peripheral nervous systems with severe and progressive neurological symptoms caused by the deficiency of Arylsulfatase A. Deficiency of this enzyme is also found in apparently healthy individuals, a condition for which the term pseudodeficiency is introduced. ARSA forms dimers after receiving three N-linked oligosaccharides in the endoplasmic reticulum, and then the dimers are transported to the Golgi where they receive mannose 6-phosphate recognition markers. And thus, ARSA is transported and delivered to dense lysosomes in a mannose 6-phosphate receptor-dependent manner. It has been shown that within the lysosomes, the ARSA dimers can oligomerize to an octamer in a pH-dependent manner. The ARSA deficiency leads to metachromatic leucodystrophy (MLD), a lysosomal storage disorder associated with severe and progressive demyelination in he central and peripheral nervous system. Additionally, the serum level of arylsulfatase A might be helpful in diagnosis of lung and central nervous system cancer.