|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Mouse CAXII ORF mammalian expression plasmid, C-GFPSpark tag||MG50014-ACG|
|Mouse CAXII ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50014-ACR|
|Mouse CAXII ORF mammalian expression plasmid, C-Flag tag||MG50014-CF|
|Mouse CAXII ORF mammalian expression plasmid, C-His tag||MG50014-CH|
|Mouse CAXII ORF mammalian expression plasmid, C-Myc tag||MG50014-CM|
|Mouse CAXII ORF mammalian expression plasmid, C-HA tag||MG50014-CY|
|Mouse CAXII Gene cDNA clone plasmid||MG50014-M|
|Mouse CAXII ORF mammalian expression plasmid, N-Flag tag||MG50014-NF|
|Mouse CAXII ORF mammalian expression plasmid, N-His tag||MG50014-NH|
|Mouse CAXII ORF mammalian expression plasmid, N-Myc tag||MG50014-NM|
|Mouse CAXII ORF mammalian expression plasmid, N-HA tag||MG50014-NY|
|Mouse CAXII natural ORF mammalian expression plasmid||MG50014-UT|
|Learn more about expression Vectors|
Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes first discovered in 1933 that catalyze the reversible hydration of carbon dioxide. CAs participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. CA12, also known as Car12 and carbonic anhydrase XII, is a type I membrane enzyme of an N-terminal extracellular catalytic domain, a membrane-spanning α-helix, and a small intracellular C-terminal domain. It is highly expressed in colon, kidney, prostate, intestine and activated lymphocytes and moderately expressed in pancreas, ovary, and testis. Overexpression of the CA12 is observed in certain human cancers and is used as a tumor marker. rmCA12 corresponds to the extracellular domain and has both carbonic anhydrase activity and esterase activity.