|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Mouse ASAH2 ORF mammalian expression plasmid, C-GFPSpark tag||MG50006-ACG|
|Mouse ASAH2 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50006-ACR|
|Mouse ASAH2 ORF mammalian expression plasmid, C-Flag tag||MG50006-CF|
|Mouse ASAH2 ORF mammalian expression plasmid, C-His tag||MG50006-CH|
|Mouse ASAH2 ORF mammalian expression plasmid, C-Myc tag||MG50006-CM|
|Mouse ASAH2 ORF mammalian expression plasmid, C-HA tag||MG50006-CY|
|Mouse ASAH2 Gene cDNA clone plasmid||MG50006-M|
|Mouse ASAH2 ORF mammalian expression plasmid, N-Flag tag||MG50006-NF|
|Mouse ASAH2 ORF mammalian expression plasmid, N-His tag||MG50006-NH|
|Mouse ASAH2 ORF mammalian expression plasmid, N-Myc tag||MG50006-NM|
|Mouse ASAH2 ORF mammalian expression plasmid, N-HA tag||MG50006-NY|
|Mouse ASAH2 natural ORF mammalian expression plasmid||MG50006-UT|
|Learn more about expression Vectors|
ASAH2 (N-acylsphingosine amidohydrolase 2), also known as neutral ceramidase, is a type II integral membrane protein that can be cleaved to produce a soluble secreted protein. The enzyme is abundant in the brush border membranes of the intestine, and also expressed in several tissues such as kidney, brain and liver. The primary structure of ASAH2/neutral ceramidase is highly conserved from bacteria to humans, however, there is a clear difference in the molecular architecture. The murine ASAH2 possesses ‘amucin box’, a Ser/Thr/Pro-rich domain glycosylated with O-glycans which is necessary to retain the enzyme on the plasma membrane as a type II integral protein. The major physiological function of ASAH2/neutral ceramidase is the metabolism of dietary sphingolipids, and thus plays a role in the generation of messenger molecules such as sphingosine and sphingosine 1-phosphate.