|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.
|Human GLIPR1 ORF mammalian expression plasmid, C-GFPSpark tag||HG10873-ACG|
|Human GLIPR1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10873-ACR|
|Human GLIPR1 ORF mammalian expression plasmid, C-Flag tag||HG10873-CF|
|Human GLIPR1 ORF mammalian expression plasmid, C-His tag||HG10873-CH|
|Human GLIPR1 ORF mammalian expression plasmid, C-Myc tag||HG10873-CM|
|Human GLIPR1 ORF mammalian expression plasmid, C-HA tag||HG10873-CY|
|Human GLIPR1 Gene cDNA clone plasmid||HG10873-M|
|Human GLIPR1 ORF mammalian expression plasmid, Flag tag||HG10873-M-F|
|Human GLIPR1 ORF mammalian expression plasmid, N-Flag tag||HG10873-NF|
|Human GLIPR1 ORF mammalian expression plasmid, N-His tag||HG10873-NH|
|Human GLIPR1 ORF mammalian expression plasmid, N-Myc tag||HG10873-NM|
|Human GLIPR1 ORF mammalian expression plasmid, N-HA tag||HG10873-NY|
|Human GLIPR1 natural ORF mammalian expression plasmid||HG10873-UT|
|Learn more about expression Vectors|
Glioma pathogenesis-related protein 1, also known as Protein RTVP-1, GLIPR1 and GLIPR, is a single-pass membrane protein which belongs to the CRISP family. GLIPR1 / RTVP-1 was expressed in high levels in glioblastomas, whereas its expression in low-grade astrocytomas and normal brains was very low. Transfection of glioma cells with small interfering RNAs targeting GLIPR1 / RTVP-1 decreased cell proliferation in all the cell lines examined and induced cell apoptosis in some of them. Overexpression of GLIPR1 / RTVP-1 increased astrocyte and glioma cell proliferation and the anchorage-independent growth of the cells. In addition, overexpression of GLIPR1 / RTVP-1 rendered glioma cells more resistant to the apoptotic effect of tumor necrosis factor-related apoptosis-inducing ligand and serum deprivation. GLIPR1 / RTVP-1 regulated the invasion of glioma cells was evident by their enhanced migration through Matrigel and by their increased invasion in a spheroid confrontation assay. The increased invasive potential of the GLIPR1 / RTVP-1 overexpressors was also shown by the increased activity of matrix metalloproteinase 2 in these cells. The expression of GLIPR1 / RTVP-1 is correlated with the degree of malignancy of astrocytic tumors and that GLIPR1 / RTVP-1 is involved in the regulation of the growth, survival, and invasion of glioma cells. GLIPR1 / RTVP-1 is a potential therapeutic target in gliomas.