|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.
|Human IL32 ORF mammalian expression plasmid, C-GFPSpark tag||HG10860-ACG|
|Human IL32 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10860-ACR|
|Human IL32 ORF mammalian expression plasmid, C-Flag tag||HG10860-CF|
|Human IL32 ORF mammalian expression plasmid, C-His tag||HG10860-CH|
|Human IL32 ORF mammalian expression plasmid, C-Myc tag||HG10860-CM|
|Human IL32 ORF mammalian expression plasmid, C-HA tag||HG10860-CY|
|Human IL32 Gene cDNA clone plasmid||HG10860-G|
|Human IL32 ORF mammalian expression plasmid, N-Flag tag||HG10860-NF|
|Human IL32 ORF mammalian expression plasmid, N-His tag||HG10860-NH|
|Human IL32 ORF mammalian expression plasmid, N-Myc tag||HG10860-NM|
|Human IL32 ORF mammalian expression plasmid, N-HA tag||HG10860-NY|
|Human IL32 natural ORF mammalian expression plasmid||HG10860-UT|
|Learn more about expression Vectors|
IL-32 is a recently discovered cytokine that induces various proinflammatory cytokines (TNF-alpha, IL-1beta, IL-6) and chemokines in both human and mouse cells through the NF-kappaB and p38 MAPK inflammatory signal pathways. It is regulated robustly by other major proinflammatory cytokines, and is crucial to inflammation and immune responses. Four of the IL-32 isoforms (alpha, beta, gamma and delta) are the most representative IL-32 transcripts, and gamma isoform of IL-32 is the most active, although all isoforms are biologically active. IL-32, a cytokine produced mainly by T, natural killer, and epithelial cells induces significant amounts of TNFalpha and MIP-2 and increases the production of both cytokines in a dose-dependent manner. IL-32 has been implicated in inflammatory disorders, mycobacterium tuberculosis infections, inflammatory bowel disease, and influenza A virus infection, as well as in some autoimmune diseases, such as rheumatoid arthritis, ulcerative colitis and in human stomach cancer, human lung cancer and breast cancer tissues. Thus, IL-32 expression might be valuable as a biomarker for cancer.