|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human PSME1 ORF mammalian expression plasmid, C-GFPSpark tag||HG14654-ACG|
|Human PSME1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG14654-ACR|
|Human PSME1 ORF mammalian expression plasmid, N-GFPSpark tag||HG14654-ANG|
|Human PSME1 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||HG14654-ANR|
|Human PSME1 ORF mammalian expression plasmid, C-Flag tag||HG14654-CF|
|Human PSME1 ORF mammalian expression plasmid, C-His tag||HG14654-CH|
|Human PSME1 ORF mammalian expression plasmid, C-Myc tag||HG14654-CM|
|Human PSME1 ORF mammalian expression plasmid, C-HA tag||HG14654-CY|
|Human PSME1 Gene cDNA clone plasmid||HG14654-G|
|Human PSME1 ORF mammalian expression plasmid, N-Flag tag||HG14654-NF|
|Human PSME1 ORF mammalian expression plasmid, N-His tag||HG14654-NH|
|Human PSME1 ORF mammalian expression plasmid, N-Myc tag||HG14654-NM|
|Human PSME1 ORF mammalian expression plasmid, N-HA tag||HG14654-NY|
|Human PSME1 natural ORF mammalian expression plasmid||HG14654-UT|
|Learn more about expression Vectors|
PA28A, also known as PSME1, is a subunit of proteasome. The 26S proteasome multicatalytic proteinase complex has a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. The immunoproteasome contains an alternate regulator, referred to as the 11S regulator or PA28, that replaces the 19S regulator. Three subunits (alpha, beta and gamma) of the 11S regulator have been identified. PSME1 gene encodes the alpha subunit of the 11S regulator, one of the two 11S subunits that is induced by gamma-interferon. Three alpha and three beta subunits combine to form a heterohexameric ring.