|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human PKM ORF mammalian expression plasmid, C-GFPSpark tag||HG16269-ACG|
|Human PKM ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG16269-ACR|
|Human PKM ORF mammalian expression plasmid, N-GFPSpark tag||HG16269-ANG|
|Human PKM ORF mammalian expression plasmid, N-OFPSpark / RFP tag||HG16269-ANR|
|Human PKM ORF mammalian expression plasmid, C-Flag tag||HG16269-CF|
|Human PKM ORF mammalian expression plasmid, C-His tag||HG16269-CH|
|Human PKM ORF mammalian expression plasmid, C-Myc tag||HG16269-CM|
|Human PKM ORF mammalian expression plasmid, C-HA tag||HG16269-CY|
|Human PKM Gene cDNA clone plasmid||HG16269-G|
|Human PKM ORF mammalian expression plasmid, N-Flag tag||HG16269-NF|
|Human PKM ORF mammalian expression plasmid, N-His tag||HG16269-NH|
|Human PKM ORF mammalian expression plasmid, N-Myc tag||HG16269-NM|
|Human PKM ORF mammalian expression plasmid, N-HA tag||HG16269-NY|
|Human PKM natural ORF mammalian expression plasmid||HG16269-UT|
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Pyruvate kinase isozymes M2 also known as pyruvate kinase muscle isozyme 2 (PKM2), pyruvate kinase type K, cytosolic thyroid hormone-binding protein (CTHBP), thyroid hormone-binding protein 1 (THBP1), or opa-interacting protein 3 (OIP3), is an isoenzyme of the glycolytic enzyme pyruvate kinase. Pyruvate kinase isozymes M2 / PKM2 is a protein involved in glycolysis. The encoded protein is a pyruvate kinase that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate to ADP, generating ATP and pyruvate. PKM2 has been shown to interact with thyroid hormone and may mediate cellular metabolic effects induced by thyroid hormones. PKM2 has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Several alternatively spliced transcript variants encoding a few distinct isoforms have been reported. PKM2 functions as a glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. PKM2 may stimulates POU5F1-mediated transcriptional activation. This protein Plays a general role in caspase independent cell death of tumor cells. The ratio betwween the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms of PKM2 contributes to the control of glycolysis and is important for tumor cell proliferation and survival.