|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein.
Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems.
|Human EGFL7 ORF mammalian expression plasmid, C-GFPSpark tag||HG11979-ACG|
|Human EGFL7 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG11979-ACR|
|Human EGFL7 ORF mammalian expression plasmid, C-Flag tag||HG11979-CF|
|Human EGFL7 ORF mammalian expression plasmid, C-His tag||HG11979-CH|
|Human EGFL7 ORF mammalian expression plasmid, C-Myc tag||HG11979-CM|
|Human EGFL7 ORF mammalian expression plasmid, C-HA tag||HG11979-CY|
|Human EGFL7 Gene cDNA clone plasmid||HG11979-G|
|Human EGFL7 ORF mammalian expression plasmid, N-Flag tag||HG11979-NF|
|Human EGFL7 ORF mammalian expression plasmid, N-His tag||HG11979-NH|
|Human EGFL7 ORF mammalian expression plasmid, N-Myc tag||HG11979-NM|
|Human EGFL7 ORF mammalian expression plasmid, N-HA tag||HG11979-NY|
|Human EGFL7 natural ORF mammalian expression plasmid||HG11979-UT|
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Epidermal growth factor-like protein 7, also known as EGF-like protein 7, Multiple epidermal growth factor-like domains protein 7, Multiple EGF-like domains protein 7, Vascular endothelial statin, NOTCH4-like protein and EGFL7, is a secreted protein which contains two EGF-like domains and one EMI domain. EGFL7 was identified by a number of groups as a putative secreted factor produced by the vascular endothelial cells (ECs). EGFL7 is described as a novel endothelial cell-derived factor involved in the regulation of the spatial arrangement of cells during vascular tube assembly. With its impact on tubulogenesis and vessel shape EGFL7 joined the large family of molecules governing blood vessel formation. EGFL7 regulates midline angioblast migration in zebrafish embryos-a key step in vascular tubulogenesis. EGFL7 is tightly associated with the extracellular matrix (ECM), and it supports EC migration either as a single factor or in combination with other ECM molecules. EGFL7 provides a proper microenvironment for endothelial cell migration, thereby enabling accurate patterning. Our study indicates that the molecular composition of the ECM influences vascular morphogenesis. EGFL7 also regulates vascular tubulogenesis. It inhibits platelet-derived growth factor (PDGF)-BB-induced smooth muscle cell migration and promotes endothelial cells adhesion to the substrate.