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Human IRAK4 Gene cDNA Clone (full-length ORF Clone), expression ready, C-HA-tagged

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IRAK4cDNA Clone Product Information
Gene_bank_ref_id:NM_016123.3
cDNA Size:1383
cDNA Description:ORF Clone of Homo sapiens interleukin-1 receptor-associated kinase 4 DNA.
Gene Synonym:IPD1, REN64, NY-REN-64, IRAK4
Species:Human
Vector:pCMV3-C-HA
Restriction Site:
Tag Sequence:HA Tag Sequence: TATCCTTACGACGTGCCTGACTACGCC
Sequence Description:
Shipping_carrier:Each tube contains approximately 10 μg of lyophilized plasmid.
Storage:The lyophilized plasmid can be stored at ambient temperature for three months.
pCMV3-C-HA Vector Information
 
Vector Name pCMV3-C-HA
Vector Size 6161bp
Vector Type Mammalian Expression Vector
Expression Method Constiutive, Stable / Transient
Promoter CMV
Antibiotic Resistance Kanamycin
Selection In Mammalian Cells Hygromycin
Protein Tag HA
Sequencing Primer Forward:T7(TAATACGACTCACTATAGGG)
Reverse:BGH(TAGAAGGCACAGTCGAGG)

pCMV3-C-HA Physical Map
Schematic of pCMV3-C-HA Multiple Cloning Sites

HA Tag Info

Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.

The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.

Human IRAK4 Gene cDNA Clone (full-length ORF Clone), expression ready, C-HA-tagged on other vectors
Human IRAK4 Gene cDNA Clone (full-length ORF Clone), expression ready, C-GFPSpark-taggedHG10735-ACG$325
Human IRAK4 Gene cDNA Clone (full-length ORF Clone), expression ready, C-OFPSpark tagHG10735-ACR$325
Human IRAK4 Gene cDNA Clone (full-length ORF Clone), expression ready, N-GFPSpark-taggedHG10735-ANG$325
Human IRAK4 Gene cDNA Clone (full-length ORF Clone), expression ready, N-OFPSpark tagHG10735-ANR$325
Human IRAK4 Gene cDNA Clone (full-length ORF Clone), expression ready, C-FLAG-taggedHG10735-CF$295
Human IRAK4 Gene cDNA Clone (full-length ORF Clone), expression ready, C-His-taggedHG10735-CH$295
Human IRAK4 Gene cDNA Clone (full-length ORF Clone), expression ready, C-Myc-taggedHG10735-CM$295
Human IRAK4 Gene cDNA Clone (full-length ORF Clone), expression ready, C-HA-taggedHG10735-CY$295
Human IRAK4 Gene cDNA Clone (full-length ORF Clone)HG10735-M$95
Human IRAK4 Gene cDNA Clone (full-length ORF Clone) expression ready, FLAG-taggedHG10735-M-F$295
Human IRAK4 Gene cDNA Clone (full-length ORF Clone) expression ready, untaggedHG10735-M-N$295
Human IRAK4 Gene cDNA Clone (full-length ORF Clone), expression ready, N-FLAG-taggedHG10735-NF$295
Human IRAK4 Gene cDNA Clone (full-length ORF Clone), expression ready, N-His-taggedHG10735-NH$295
Human IRAK4 Gene cDNA Clone (full-length ORF Clone), expression ready, N-Myc-taggedHG10735-NM$295
Human IRAK4 Gene cDNA Clone (full-length ORF Clone), expression ready, N-HA-taggedHG10735-NY$295
Human IRAK4 Gene cDNA Clone (full-length ORF Clone), expression ready, untaggedHG10735-UT$295
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Background

Interleukin-1 receptor-associated kinase 4, also known as Renal carcinoma antigen NY-REN-64, IRAK-4 and IRAK4, is a member of the protein kinase superfamily, TKL Ser/Thr protein kinase family and Pelle subfamily. IRAK4 contains one death domain and one protein kinase domain. IRAK4 is required for the efficient recruitment of IRAK1 to the IL-1 receptor complex following IL-1 engagement, triggering intracellular signaling cascades leading to transcriptional up-regulation and mRNA stabilization. It also phosphorylates IRAK1. A member of the IL-1 receptor (IL-1R)-associated kinase (IRAK) family, IRAK4, has been shown to play an essential role in Toll-like receptor (TLR)-mediated signaling. IL-1-mediated IRAK4 kinase activity in T cells is essential for induction of IL-23R expression, Th17 differentiation, and autoimmune disease. Pharmacological blocking of IRAK4 kinase activity will retain some levels of host defence, while reducing the levels and duration of inflammatory responses, which should provide beneficial therapies for sepsis and chronic inflammatory diseases. Defects in IRAK4 are the cause of recurrent isolated invasive pneumococcal disease type 1 (IPD1) which is defined as two episodes of IPD occurring at least 1 month apart, whether caused by the same or different serotypes or strains. Recurrent IPD occurs in at least 2% of patients in most series, making IPD the most important known risk factor for subsequent IPD. Defects in IRAK4 are also the cause of IRAK4 deficiency which causes extracellular pyogenic bacterial and fungal infections in otherwise healthy children.

References
  • Strelow,A. et al., 2003, FEBS Lett. 547 (1-3):157-61.
  • Kim,T.W. et al., 2007, J Exp Med. 204 (5):1025-36.
  • Trumstedt,C. et al., 2007, J Leukoc Biol. 81 (6):1591-8.
  • Li,X. et al., 2008, Eur J Immunol. 38 (3):614-8.
  • Staschke,K.A. et al., 2009, J Immunol. 183 (1): 568-77. 
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    Catalog:HG10735-CY
    List Price: $295.00  (Save $0.00)
    Price:$295.00      [How to order]
    Availability2-3 weeks
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