|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human CADM4 ORF mammalian expression plasmid, C-GFPSpark tag||HG16033-ACG|
|Human CADM4 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG16033-ACR|
|Human CADM4 ORF mammalian expression plasmid, C-Flag tag||HG16033-CF|
|Human CADM4 ORF mammalian expression plasmid, C-His tag||HG16033-CH|
|Human CADM4 ORF mammalian expression plasmid, C-Myc tag||HG16033-CM|
|Human CADM4 ORF mammalian expression plasmid, C-HA tag||HG16033-CY|
|Human CADM4 Gene cDNA clone plasmid||HG16033-G|
|Human CADM4 ORF mammalian expression plasmid, N-Flag tag||HG16033-NF|
|Human CADM4 ORF mammalian expression plasmid, N-His tag||HG16033-NH|
|Human CADM4 ORF mammalian expression plasmid, N-Myc tag||HG16033-NM|
|Human CADM4 ORF mammalian expression plasmid, N-HA tag||HG16033-NY|
|Human CADM4 natural ORF mammalian expression plasmid||HG16033-UT|
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Immunoglobulin superfamily member 4C (IGSF4C), also known as CADM4 or NECL-4, is an immunoglobulin (Ig) superfamily molecule showing significant homology with a lung tumor suppressor, TSLC1. CADM4/IGSF4C/NECL-4 protein is mainly expressed in the kidney, bladder, and prostate in addition to the brain. Experiments have reported the biological significance of CADM4/IGSF4C/NECL-4 in the urinary tissues. An immunohistochemical study reveals that CADM4 is expressed at the cell-cell attachment sites in the renal tubules, the transitional epithelia of the bladder, and the glandular epithelia of the prostate. IGSF4-immunoreactivity (IR) was observed diffusely in the telencephalic wall, whereas it became rather confined to the subplate, the cortical plate and the subventricular zone as the development proceeded. IGSF4-IR gradually decreased after birth and disappeared in adulthood. IGSF4 remained at low levels throughout embryonic stage, whereas it increased after birth. These spatiotemporal patterns of the expression suggest that IGSF4 plays crucial roles in the development of both telencephalon and cerebellum. CADM4/IGSF4C/NECL-4 is ectopically expressed in adult T-cell leukemia (ATL) cells, providing not only a diagnostic marker for ATL, but also a possible therapeutic target against its invasion. The distinct roles of CADM4/IGSF4C/NECL-4 in the oncogenesis of carcinomas and ATL could be due to tissue-specific differences in the downstream cascades, and is a novel concept with respect to cell adhesion in human oncogenesis.