|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human ENTPD1 ORF mammalian expression plasmid, C-GFPSpark tag||HG16020-ACG|
|Human ENTPD1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG16020-ACR|
|Human ENTPD1 ORF mammalian expression plasmid, C-Flag tag||HG16020-CF|
|Human ENTPD1 ORF mammalian expression plasmid, C-His tag||HG16020-CH|
|Human ENTPD1 ORF mammalian expression plasmid, C-Myc tag||HG16020-CM|
|Human ENTPD1 ORF mammalian expression plasmid, C-HA tag||HG16020-CY|
|Human ENTPD1 Gene cDNA clone plasmid||HG16020-G|
|Human ENTPD1 ORF mammalian expression plasmid, N-Flag tag||HG16020-NF|
|Human ENTPD1 ORF mammalian expression plasmid, N-His tag||HG16020-NH|
|Human ENTPD1 ORF mammalian expression plasmid, N-Myc tag||HG16020-NM|
|Human ENTPD1 ORF mammalian expression plasmid, N-HA tag||HG16020-NY|
|Human ENTPD1 natural ORF mammalian expression plasmid||HG16020-UT|
|Learn more about expression Vectors|
CD39, also known as ENTPD1, belongs to the GDA1/CD39 NTPase family. It is expressed primarily on activated lymphoid cells and can also be detected in endothelial tissues. The vascular isoform and the placental isoform II are present in both placenta and umbilical vein, whereas placental isoform I is present in placenta only. CD39 can hydrolyze both nucleoside triphosphates and diphosphates. It is the dominant ecto nucleotidase of vascular and placental trophoblastic tissues and appears to modulate the functional expression of type 2 purinergic (P2) G protein coupled receptors (GPCRs). CD39 transgenic mice exhibit impaired platelet aggregation, prolonged bleeding times, and resistance to systemic thromboembolism. There is a correlation between ATP hydrolysis and triglycerides in patients with chronic heart disease, suggesting a relationship between ATP diphosphohydrolase and thrombogenesis. In the nervous system, CD39 could hydrolyze ATP and other nucleotides to regulate purinergic neurotransmission.