|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.
|Human MEK1 ORF mammalian expression plasmid, C-GFPSpark tag||HG10661-ACG|
|Human MEK1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10661-ACR|
|Human MEK1 ORF mammalian expression plasmid, N-GFPSpark tag||HG10661-ANG|
|Human MEK1 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||HG10661-ANR|
|Human MEK1 ORF mammalian expression plasmid, C-Flag tag||HG10661-CF|
|Human MEK1 ORF mammalian expression plasmid, C-His tag||HG10661-CH|
|Human MEK1 ORF mammalian expression plasmid, C-Myc tag||HG10661-CM|
|Human MEK1 ORF mammalian expression plasmid, C-HA tag||HG10661-CY|
|Human MEK1 Gene cDNA clone plasmid||HG10661-M|
|Human MEK1 natural ORF mammalian expression plasmid||HG10661-M-N|
|Human MEK1 ORF mammalian expression plasmid, N-Flag tag||HG10661-NF|
|Human MEK1 ORF mammalian expression plasmid, N-His tag||HG10661-NH|
|Human MEK1 ORF mammalian expression plasmid, N-Myc tag||HG10661-NM|
|Human MEK1 ORF mammalian expression plasmid, N-HA tag||HG10661-NY|
|Human MEK1 natural ORF mammalian expression plasmid||HG10661-UT|
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MEK1, also known as MAP2K1 and MKK1, is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. MEK1 is widely expressed, with extremely low levels in brain. It lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, MEK1 is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development. Binding extracellular ligands such as growth factors, cytokines and hormones to their cell-surface receptors activates RAS and this initiates RAF1 activation. RAF1 then further activates the dual-specificity protein kinases MAP2K1 and MEK2. MEK1 has been shown to export PPARG from the nucleus. The MAPK cascade is also involved in the regulation of endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC), as well as in the fragmentation of the Golgi apparatus during mitosis. MKK1 catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Defects in MEK1 can cause cardiofaciocutaneous syndrome.