|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human FZD1 ORF mammalian expression plasmid, C-GFPSpark tag||HG15229-ACG|
|Human FZD1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG15229-ACR|
|Human FZD1 ORF mammalian expression plasmid, C-Flag tag||HG15229-CF|
|Human FZD1 ORF mammalian expression plasmid, C-His tag||HG15229-CH|
|Human FZD1 ORF mammalian expression plasmid, C-Myc tag||HG15229-CM|
|Human FZD1 ORF mammalian expression plasmid, C-HA tag||HG15229-CY|
|Human FZD1 Gene cDNA clone plasmid||HG15229-G|
|Human FZD1 ORF mammalian expression plasmid, N-Flag tag||HG15229-NF|
|Human FZD1 ORF mammalian expression plasmid, N-His tag||HG15229-NH|
|Human FZD1 ORF mammalian expression plasmid, N-Myc tag||HG15229-NM|
|Human FZD1 ORF mammalian expression plasmid, N-HA tag||HG15229-NY|
|Human FZD1 natural ORF mammalian expression plasmid||HG15229-UT|
|Learn more about expression Vectors|
Frizzled-1, also known as FZD1, belongs to the G-protein coupled receptor Fz/Smo family. FZD1 contains a signal peptide, a cysteine-rich domain in the N-terminal extracellular region, 7 transmembrane domains, and a C-terminal PDZ domain-binding motif. FZD1 is expressed in adult heart, placenta, lung, kidney, pancreas, prostate, and ovary and in fetal lung and kidney. Frizzled is a family of G protein-coupled receptor proteins that serve as receptors in the Wnt signaling pathway and other signaling pathways. When activated, Frizzled leads to activation of Dishevelled in the cytosol. Frizzled proteins and the genes encoding them have been identified in an array of animals, from sponges to humans. Frizzled proteins play key roles in governing cell polarity, embryonic development, formation of neural synapses, cell proliferation, and many other processes in developing and adult organisms. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes.