|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human BPHL ORF mammalian expression plasmid, C-GFPSpark tag||HG15763-ACG|
|Human BPHL ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG15763-ACR|
|Human BPHL ORF mammalian expression plasmid, C-Flag tag||HG15763-CF|
|Human BPHL ORF mammalian expression plasmid, C-His tag||HG15763-CH|
|Human BPHL ORF mammalian expression plasmid, C-Myc tag||HG15763-CM|
|Human BPHL ORF mammalian expression plasmid, C-HA tag||HG15763-CY|
|Human BPHL Gene cDNA clone plasmid||HG15763-G|
|Human BPHL ORF mammalian expression plasmid, N-Flag tag||HG15763-NF|
|Human BPHL ORF mammalian expression plasmid, N-His tag||HG15763-NH|
|Human BPHL ORF mammalian expression plasmid, N-Myc tag||HG15763-NM|
|Human BPHL ORF mammalian expression plasmid, N-HA tag||HG15763-NY|
|Human BPHL natural ORF mammalian expression plasmid||HG15763-UT|
|Learn more about expression Vectors|
BPHL is a member of the serine protease family. BPHL is expressed large quantities in liver and kidney and in minor quantities in heart, intestine and skeletal muscle. BPHL is a specific alpha-amino acid ester hydrolase that prefers small, hydrophobic, and aromatic side chains and does not have a stringent requirement for the leaving group other than preferring a primary alcohol. It catalyzes the hydrolytic activation of amino acid ester prodrugs of nucleoside analogs such as valacyclovir and valganciclovir. BPHL also activates valacyclovir to acyclovir. It may play a role in detoxification processes.