|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.
|Human MMP-1 ORF mammalian expression plasmid, C-GFPSpark tag||HG10532-ACG|
|Human MMP-1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10532-ACR|
|Human MMP-1 ORF mammalian expression plasmid, C-Flag tag||HG10532-CF|
|Human MMP-1 ORF mammalian expression plasmid, C-His tag||HG10532-CH|
|Human MMP-1 ORF mammalian expression plasmid, C-Myc tag||HG10532-CM|
|Human MMP-1 ORF mammalian expression plasmid, C-HA tag||HG10532-CY|
|Human MMP-1 Gene cDNA clone plasmid||HG10532-M|
|Human MMP-1 ORF mammalian expression plasmid, N-Flag tag||HG10532-NF|
|Human MMP-1 ORF mammalian expression plasmid, N-His tag||HG10532-NH|
|Human MMP-1 ORF mammalian expression plasmid, N-Myc tag||HG10532-NM|
|Human MMP-1 ORF mammalian expression plasmid, N-HA tag||HG10532-NY|
|Human MMP-1 natural ORF mammalian expression plasmid||HG10532-UT|
|Learn more about expression Vectors|
MMP1, also known as MMP-1, contains 4 hemopexin-like domains and is a member of the matrix metalloproteinase (MMP) family. Matrix metalloproteases, also called matrixins, are zinc-dependent endopeptidases that are the major proteases involved in ECM degradation. MMPs are capable of degrading a wide range of extracellular molecules and a number of bioactive molecules. MMP activity is regulated by two major endogenous inhibitors: alpha2-macroglobulin and tissue inhibitors of metalloproteases (TIMPs). MMPs play a central role in cell proliferation, migration, differentiation, angiogenesis, apoptosis and host defences. Dysregulatoin of MMPs has been implicated in many diseases including arthritis, chronic ulcers, encephalomyelitis and cancer. Tumour metastasis is a multistep process involving the dessemination of tumor cells from the primary tumor to secondarys at a distant organ or tissue. One of the first steps in metastasis is the degradation of the basement membrane, a process in which MMPs have been implicated. MMPs are secreted by tumor cells themselves or by surrounding stromal cells stimulated by the nearby tumor. Numerous studies have linked altered MMP expression in different human cancers with poor disease prognosis. MMP-1, -2, -3, -7, -9, -13 and -14 all have elevated expression in primary tumors and/or metastases. MMP-1 cleaves collagens of types I, II, and III at one site in the helical domain. It also cleaves collagens of types VII and X. In case of HIV infection, MMP1 interacts and cleaves the secreted viral Tat protein, leading to a decrease in neuronal Tat's mediated neurotoxicity.