|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human ARF3 ORF mammalian expression plasmid, C-GFPSpark tag||HG14967-ACG|
|Human ARF3 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG14967-ACR|
|Human ARF3 ORF mammalian expression plasmid, C-Flag tag||HG14967-CF|
|Human ARF3 ORF mammalian expression plasmid, C-His tag||HG14967-CH|
|Human ARF3 ORF mammalian expression plasmid, C-Myc tag||HG14967-CM|
|Human ARF3 ORF mammalian expression plasmid, C-HA tag||HG14967-CY|
|Human ARF3 Gene cDNA clone plasmid||HG14967-G|
|Human ARF3 ORF mammalian expression plasmid, N-Flag tag||HG14967-NF|
|Human ARF3 ORF mammalian expression plasmid, N-His tag||HG14967-NH|
|Human ARF3 ORF mammalian expression plasmid, N-Myc tag||HG14967-NM|
|Human ARF3 ORF mammalian expression plasmid, N-HA tag||HG14967-NY|
|Human ARF3 natural ORF mammalian expression plasmid||HG14967-UT|
|Learn more about expression Vectors|
ARF3, also known as ADP-ribosylation factor 3, belongs to the RAS superfamily. Members of this family include ARF1, ARF2, ARF3, ARF4, ARF5 and ARF6. ARF3 gene is a member of the human ARF gene family. These genes encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. ARF3 functions as an allosteric activator of the cholera toxin subunit, an ADP-ribosyltransferase. It is involved in protein trafficking and may modulate vesicle budding and uncoating within the Golgi apparatus.