|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.
|Human HAPLN1 ORF mammalian expression plasmid, C-GFPSpark tag||HG10323-ACG|
|Human HAPLN1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10323-ACR|
|Human HAPLN1 ORF mammalian expression plasmid, C-Flag tag||HG10323-CF|
|Human HAPLN1 ORF mammalian expression plasmid, C-His tag||HG10323-CH|
|Human HAPLN1 ORF mammalian expression plasmid, C-Myc tag||HG10323-CM|
|Human HAPLN1 ORF mammalian expression plasmid, C-HA tag||HG10323-CY|
|Human HAPLN1 Gene cDNA clone plasmid||HG10323-M|
|Human HAPLN1 ORF mammalian expression plasmid, Flag tag||HG10323-M-F|
|Human HAPLN1 natural ORF mammalian expression plasmid||HG10323-M-N|
|Human HAPLN1 ORF mammalian expression plasmid, N-Flag tag||HG10323-NF|
|Human HAPLN1 ORF mammalian expression plasmid, N-His tag||HG10323-NH|
|Human HAPLN1 ORF mammalian expression plasmid, N-Myc tag||HG10323-NM|
|Human HAPLN1 ORF mammalian expression plasmid, N-HA tag||HG10323-NY|
|Human HAPLN1 natural ORF mammalian expression plasmid||HG10323-UT|
|Learn more about expression Vectors|
Hyaluronan (HA) is a high MW glycosaminoglycan significantly involved in the formation and stability of extracellular matrix via its association with specific HA-binding proteins. HAPLN1, also known as CRTL1 (Cartilage Link Protein 1, cLP ) and link protein, is a member of HA-binding protein (hyaladherins) family, and contains a common structural domain of about 100 amino acids that is termed a Link module with two α-helices and two antiparallel β-sheets. HAPLN1/CRTL1 stabilizes the interaction between hyaluronan (HA) and versican, two extracellular matrix components essential for cardiac development. Link module superfamily can be divided into three subgroups, and the HAPLN family are C domain-type proteins that have an extended structure with one N-terminal V-type Ig-like domain followed by two link modules. In cartilage, aggrecan forms - cLP stabilized aggregates with HA that provides the tissue with its load bearing properties. HAPLN1 is a component of follicular matrix, was shown to enhance cumulus-oocyte complex (COC) expansion in vitro. HAPLN1 may promote periovulatory granulosa cell survival, which would facilitate their differentiation into luteal cells.