|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.
|Human Cadherin-12 ORF mammalian expression plasmid, C-GFPSpark tag||HG10317-ACG|
|Human Cadherin-12 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10317-ACR|
|Human Cadherin-12 ORF mammalian expression plasmid, C-Flag tag||HG10317-CF|
|Human Cadherin-12 ORF mammalian expression plasmid, C-His tag||HG10317-CH|
|Human Cadherin-12 ORF mammalian expression plasmid, C-Myc tag||HG10317-CM|
|Human Cadherin-12 ORF mammalian expression plasmid, C-HA tag||HG10317-CY|
|Human Cadherin-12 Gene cDNA clone plasmid||HG10317-M|
|Human Cadherin-12 ORF mammalian expression plasmid, N-Flag tag||HG10317-NF|
|Human Cadherin-12 ORF mammalian expression plasmid, N-His tag||HG10317-NH|
|Human Cadherin-12 ORF mammalian expression plasmid, N-Myc tag||HG10317-NM|
|Human Cadherin-12 ORF mammalian expression plasmid, N-HA tag||HG10317-NY|
|Human Cadherin-12 natural ORF mammalian expression plasmid||HG10317-UT|
|Learn more about expression Vectors|
Classic Cadherins represent a family of calcium-dependent homophilic cell-cell adhesion molecules. They confer strong adhesiveness to animal cells when they are anchored to the actin cytoskeleton via their cytoplasmic binding partners, catenins. The cadherin/catenin adhesion system plays key roles in the morphogenesis and function of the vertebrate and invertebrate nervous systems. Furthermore, this system is involved in synaptic plasticity. Recent studies on the role of individual cadherin subtypes at synapses indicate that individual cadherin subtypes play their own unique role to regulate synaptic activities. Type II (atypical) cadherins are defined based on their lack of an HAV cell adhesion recognition sequence specific to type I cadherins. It has been observed that cells containing a specific cadherin subtype tend to cluster together to the exclusion of other types, both in cell culture and during development. Cadherin-12 also known as CDH12, is a type II classical cadherin from the cadherin superfamily of integral membrane proteins that mediate calcium-dependent cell-cell adhesion. Cadherin-12 appears to be expressed specifically in the brain and its temporal pattern of expression would be consistent with a role during a critical period of neuronal development, perhaps specifically during synaptogenesis.