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Human ADCYAP1R1 ORF mammalian expression plasmid, C-His tag

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Human ADCYAP1R1 cDNA Clone Product Information
NCBI RefSeq:BC117116
RefSeq ORF Size:1491bp
cDNA Description:Full length Clone DNA of Homo sapiens adenylate cyclase activating polypeptide 1 (pituitary) receptor type I with C terminal His tag.
Gene Synonym:PAC1, PAC1R, PACAPR, PACAPRI
Species:Human
Vector:pCMV3-C-His
Plasmid:
Restriction Site:
Tag Sequence:His Tag Sequence: CACCATCACCACCATCATCACCACCATCAC
Sequence Description:
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
Storage:The lyophilized plasmid can be stored at room temperature for three months.
His Tag Info

A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein.

Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokarfyotic expression systems.

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References
  • Solano RM. et al., 1996, Endocrinology. 137 (7): 2815-22.
  • Miampamba M. et al., 2002, Regul Pept. 105 (3): 145-54.
  • Brabet P. et al., 1997, Genomics. 38 (1): 100-2.
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    Catalog: HG13223-CH
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