|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human GOPC ORF mammalian expression plasmid, C-GFPSpark tag||HG14911-ACG|
|Human GOPC ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG14911-ACR|
|Human GOPC ORF mammalian expression plasmid, N-GFPSpark tag||HG14911-ANG|
|Human GOPC ORF mammalian expression plasmid, N-OFPSpark / RFP tag||HG14911-ANR|
|Human GOPC ORF mammalian expression plasmid, C-Flag tag||HG14911-CF|
|Human GOPC ORF mammalian expression plasmid, C-His tag||HG14911-CH|
|Human GOPC ORF mammalian expression plasmid, C-Myc tag||HG14911-CM|
|Human GOPC ORF mammalian expression plasmid, C-HA tag||HG14911-CY|
|Human GOPC Gene cDNA clone plasmid||HG14911-G|
|Human GOPC ORF mammalian expression plasmid, N-Flag tag||HG14911-NF|
|Human GOPC ORF mammalian expression plasmid, N-His tag||HG14911-NH|
|Human GOPC ORF mammalian expression plasmid, N-Myc tag||HG14911-NM|
|Human GOPC ORF mammalian expression plasmid, N-HA tag||HG14911-NY|
|Human GOPC natural ORF mammalian expression plasmid||HG14911-UT|
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GOPC, also known as PIST, interacts specifically with TC10 (a Rho-family small GTPase)] as a binding partner for Rhotekin. Rhotekin associates with PIST in vitro and in both polarized and non-polarized MDCK (Madin-Darby canine kidney) cells. The C-terminal SPV (Ser-Pro-Val) motif of Rhotekin binds to the PDZ domain of PIST. The co-localization of PIST and Rhotekin at the Golgi apparatus can be detected in non-polarized fibroblast-like MDCK cells and AJs (adherens junctions) in the fully polarized cells. PIST and Rhotekin are recruited from the cytosol to AJs as the cell becomes polarized. Expression of constitutively active Rho or prevention of Rhotekin-PIST interaction induced diffuse cytoplasmic distribution of Rhotekin in polarized MDCK cells. GOPC may regulate CFTR chloride currents and acid-induced ACCN3 currents by modulating cell surface expression of both channels. It may also regulate the intracellular trafficking of the ADR1B receptor. GOPC is ubiquitously expressed and its overexpression results in CFTR intracellular retention and degradation in the lysosomes.