|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein.
Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems.
|Human NCSTN ORF mammalian expression plasmid, C-GFPSpark tag||HG11183-ACG|
|Human NCSTN ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG11183-ACR|
|Human NCSTN ORF mammalian expression plasmid, C-Flag tag||HG11183-CF|
|Human NCSTN ORF mammalian expression plasmid, C-His tag||HG11183-CH|
|Human NCSTN ORF mammalian expression plasmid, C-Myc tag||HG11183-CM|
|Human NCSTN ORF mammalian expression plasmid, C-HA tag||HG11183-CY|
|Human NCSTN Gene cDNA clone plasmid||HG11183-M|
|Human NCSTN ORF mammalian expression plasmid, N-Flag tag||HG11183-NF|
|Human NCSTN ORF mammalian expression plasmid, N-His tag||HG11183-NH|
|Human NCSTN ORF mammalian expression plasmid, N-Myc tag||HG11183-NM|
|Human NCSTN ORF mammalian expression plasmid, N-HA tag||HG11183-NY|
|Human NCSTN natural ORF mammalian expression plasmid||HG11183-UT|
|Learn more about expression Vectors|
Nicastrin (NCST, or NCT), a single-pass membrane glycoprotein that harbors a large extracellular domain, is an essential component of the gamma-secretase complex. Several lines of evidence indicate that the members of these complexes could also contribute to the control of cell death. NCT controls cell death via phosphoinositide 3-kinase/Akt and p53-dependent pathways and that this function remains independent of the activity and molecular integrity of the gamma-secretase complexes. Increasing evidences have shown that Nicastrin/NCSTN plays a crucial role in gamma-cleavage of the amyloid precursor protein (APP). The glycoprotein Nicastrin is an essential component of the gamma-secretase complex, a high molecular weight complex which also contains the presenilin proteins, Aph-1 and Pen-2. The gamma-secretase complex is not only involved in APP processing but also in the processing of an increasing number of other type I integral membrane proteins. As the largest subunit of the gamma-secretase complex, Nicastrin plays a crucial role in its activation. Inhibition of NCSTN demonstrated an altered gamma-cleavage activity, suggesting its potential implication in developing Alzheimer's disease (AD). In addition, Nicastrin can function to maintain epithelial to mesenchymal transition during breast cancer progression. Anti-nicastrin polyclonal and monoclonal antibodies were able to decrease notch1 and vimentin expression and reduced the invasive capacity of breast cancer cells in vitro.