|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human LIF ORF mammalian expression plasmid, C-GFPSpark tag||HG14890-ACG|
|Human LIF ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG14890-ACR|
|Human LIF ORF mammalian expression plasmid, C-Flag tag||HG14890-CF|
|Human LIF ORF mammalian expression plasmid, C-His tag||HG14890-CH|
|Human LIF ORF mammalian expression plasmid, C-Myc tag||HG14890-CM|
|Human LIF ORF mammalian expression plasmid, C-HA tag||HG14890-CY|
|Human LIF Gene cDNA Clone (full-length ORF Clone)||HG14890-G|
|Human LIF ORF mammalian expression plasmid, N-Flag tag||HG14890-NF|
|Human LIF ORF mammalian expression plasmid, N-His tag||HG14890-NH|
|Human LIF ORF mammalian expression plasmid, N-Myc tag||HG14890-NM|
|Human LIF ORF mammalian expression plasmid, N-HA tag||HG14890-NY|
|Human LIF natural ORF mammalian expression plasmid||HG14890-UT|
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Leukemia inhibitory factor (LIF) is a pleiotropic glycoprotein belonging to the IL-6 family of cytokines. It’s involved in growth promotion and cell differentiation of different types of target cells, influence on bone metabolism, cachexia, neural development, embryogenesis and inflammation. LIF has potent proinflammatory property, being the inducer of the acute phase protein synthesis and affecting the cell recruitment into the area of damage or inflammation. LIF is also one of the cytokines that are capable to regulate the differentiation of embryonic stem cells, hematopoietic and neuronal cells. LIF binds to the specific LIF receptor (LIFR-α) which forms a heterodimer with a specific subunit common to all members of that family of receptors, the GP130 signal transducing subunit. This leads to activation of the JAK/STAT and MAPK cascades. Due to its polyfunctional activities, LIF is involved in the pathogenic events and development of many diseases of various origin.