|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein.
Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokarfyotic expression systems.
|Human PTPRJ ORF mammalian expression plasmid, C-GFPSpark tag||HG13165-ACG|
|Human PTPRJ ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG13165-ACR|
|Human PTPRJ ORF mammalian expression plasmid, C-Flag tag||HG13165-CF|
|Human PTPRJ ORF mammalian expression plasmid, C-His tag||HG13165-CH|
|Human PTPRJ ORF mammalian expression plasmid, C-Myc tag||HG13165-CM|
|Human PTPRJ ORF mammalian expression plasmid, C-HA tag||HG13165-CY|
|Human PTPRJ Gene cDNA clone plasmid||HG13165-G|
|Human PTPRJ ORF mammalian expression plasmid, N-Flag tag||HG13165-NF|
|Human PTPRJ ORF mammalian expression plasmid, N-His tag||HG13165-NH|
|Human PTPRJ ORF mammalian expression plasmid, N-Myc tag||HG13165-NM|
|Human PTPRJ ORF mammalian expression plasmid, N-HA tag||HG13165-NY|
|Human PTPRJ natural ORF mammalian expression plasmid||HG13165-UT|
|Learn more about expression Vectors|
DEP1 / PTPRJ (Receptor-type tyrosine-protein phosphatase eta) is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes, including cell growth, differentiation, mitotic cycle, and oncogenic transformation. DEP1 / PTPRJ possesses an extracellular region containing five fibronectin type III repeats, a single transmembrane region, and a single intracytoplasmic catalytic domain, and thus represents a receptor-type PTP. DEP1 / PTPRJ is present in all hematopoietic lineages, and was shown to negatively regulate T cell receptor signaling possibly through interfering with the phosphorylation of Phospholipase C Gamma 1 and Linker for Activation of T Cells. This protein can also dephosphorylate the PDGF beta receptor, and may be involved in UV-induced signal transduction. In stable MCF-7 cell lines lines, induction of DEP-1 expression inhibited breast cancer cell growth by 5-10-fold. These data describe PTPs expressed and regulated in breast cancer cell lines during differentiation and identify one PTP, DEP-1, that inhibits the growth of breast cancer cells in vitro.